The Arabidopsis nitrate transporter NRT 2.5 plays a role in nitrate acquisition and remobilization in nitrogen‐starved plants

Abstract

Summary: Nitrogen is a key mineral nutrient playing a crucial role in plant growth and development. Understanding the mechanisms of nitrate uptake from the soil and distribution through the plant in response to nitrogen starvation is an important step on the way to improve nitrogen uptake and utilization efficiency for better growth and productivity of plants, and to prevent negative effects of nitrogen fertilizers on the environment and human health. In this study, we show that Arabidopsis NITRATE TRANSPORTER 2.5 (NRT 2.5) is a plasma membrane‐localized high‐affinity nitrate transporter playing an essential role in adult plants under severe nitrogen starvation. NRT2.5 expression is induced under nitrogen starvation and NRT 2.5 becomes the most abundant transcript amongst the seven NRT 2 family members in shoots and roots of adult plants after long‐term starvation. GUS reporter analyses showed that NRT 2.5 is expressed in the epidermis and the cortex of roots at the root hair zone and in minor veins of mature leaves. Reduction of NRT 2.5 expression resulted in a decrease in high‐affinity nitrate uptake without impacting low‐affinity uptake. In the background of the high‐affinity nitrate transporter mutant nrt2.4, an nrt2.5 mutation reduced nitrate levels in the phloem of N‐starved plants further than in the single nrt2.4 mutants. Growth analyses of multiple mutants between NRT 2.1, NRT 2.2, NRT 2.4, and NRT 2.5 revealed that NRT 2.5 is required to support growth of nitrogen‐starved adult plants by ensuring the efficient uptake of nitrate collectively with NRT 2.1, NRT 2.2 and NRT 2.4 and by taking part in nitrate loading into the phloem during nitrate remobilization.