The DOI matches its Crossref record; 24 of the 24 references it cites were found in a registry or an index.
Activation of protein kinase B (Akt/RAC‐protein kinase) by cellular stress and its association with heat shock protein Hsp27
Open accessAbstract
Protein kinase B (PKB, also named as Akt or RAC‐protein kinase), that is activated by cellular stress such as heat shock and hyperosmotic treatment, was revealed to be activated by oxidative stress and by chemical stressors of CdCl 2 and NaAsO 2 by measuring the activity of the enzyme immunoprecipitated from the transfected COS‐7 cells. Upon stress treatment, a 30‐kDa phosphoprotein was co‐immunoprecipitated with PKB from the cells metabolic labeled with [32 P]orthophosphate. The phosphoprotein was identified as Hsp27, a small heat shock protein, by immunoblot analysis and co‐immunoprecipitation. The association of Hsp27 was specific to PKB as the heat shock protein was not co‐immunoprecipitated with other protein kinases such as protein kinase C and PKN. When the cells were treated with H 2 O 2, PKB was activated gradually and the association of Hsp27 with PKB increased concurrently with the enhancement of PKB activity. In heat‐shocked cells, activation of PKB and the association of Hsp27 were detected immediately after the treatment, and the association of the heat shock protein decreased while PKB kept stimulated activity when the cells were further incubated at 37°C. These results suggest that Hsp27 is involved in the activation process of PKB in the signal transduction pathway of various forms of stress.
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Cites
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Cited by
Oxidative stress, inflammation, and cancer: How are they linked?
Reuter, S., Gupta, S. C., Chaturvedi, M. M., & Aggarwal, B. B. (2010) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2010.09.006 · Cited by 5,441
Cellular response to oxidative stress: Signaling for suicide and survival*
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The activation of Akt/PKB signaling pathway and cell survival
Song, G., Ouyang, G., & Bao, S. (2005) · Journal of Cellular and Molecular Medicine · doi:10.1111/j.1582-4934.2005.tb00337.x · Cited by 1,888
The Role of Oxidative Stress in Carcinogenesis
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Cellular Stress Responses: Cell Survival and Cell Death
Fulda, S., Gorman, A. M., Hori, O., & Samali, A. (2010) · International Journal of Cell Biology · doi:10.1155/2010/214074 · Cited by 1,519
Metadata
- Authors
- Konishi, H., Matsuzaki, H., Tanaka, M., Takemura, Y., Kuroda, S., Ono, Y., & Kikkawa, U.
- Venue
- FEBS Letters
- Publisher
- Wiley
- Published
- 1997-06-30
- Volume
- 410
- Issue
- 2-3
- Pages
- 493-498
- ISSN
- 0014-5793 (ISSN-L)
- 1873-3468
- OpenAlex
- W2019872821
- Cited by
- 270
- References
- 24
- FWCI
- 3.49
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Konishi, H., Matsuzaki, H., Tanaka, M., Takemura, Y., Kuroda, S., Ono, Y., & Kikkawa, U. (1997). Activation of protein kinase B (Akt/RAC‐protein kinase) by cellular stress and its association with heat shock protein Hsp27. FEBS Letters. https://doi.org/10.1016/s0014-5793(97)00541-3
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