The DOI matches its Crossref record; 18 of the 18 references it cites were found in a registry or an index.
Ratio‐fluorescence microscopy of lipid oxidation in living cells using C11‐BODIPY581/591
Open accessAbstract
A ratio‐fluorescence assay was developed for on‐line localization and quantification of lipid oxidation in living cells. The assay explores the oxidative sensitivity of C11‐BODIPY 581/591. Upon oxidation, the fluorescence of this fluorophore shifts from red to green. The probe incorporates readily into cellular membranes and is about twice as sensitive to oxidation as arachidonic acid. Using confocal microscopy, the cumene hydroperoxide‐induced oxidation of C11‐BODIPY 581/591 was visualized at the sub‐cellular level in rat‐1 fibroblasts. Pre‐loading of the cells with tocopherol retarded this oxidation. The data demonstrate that C11‐BODIPY 581/591 is a valuable tool to quantify lipid oxidation and anti‐oxidant efficacy in single cells.
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Cited by
Forkhead transcription factor FOXO3a protects quiescent cells from oxidative stress
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Metadata
- Authors
- Pap, E. H. W., Drummen, G. P. C., Winter, V. J., Kooij, T. W. A., Rijken, P., Wirtz, K. W. A., Op den Kamp, J. A. F., Hage, W. J., & Post, J. A.
- Venue
- FEBS Letters
- Publisher
- Wiley
- Published
- 1999-06-23
- Volume
- 453
- Issue
- 3
- Pages
- 278-282
- ISSN
- 0014-5793 (ISSN-L)
- 1873-3468
- OpenAlex
- W2012326456
- Cited by
- 329
- References
- 18
- FWCI
- 4.09
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Pap, E. H. W., Drummen, G. P. C., Winter, V. J., Kooij, T. W. A., Rijken, P., Wirtz, K. W. A., Op den Kamp, J. A. F., Hage, W. J., & Post, J. A. (1999). Ratio‐fluorescence microscopy of lipid oxidation in living cells using C11‐BODIPY581/591. FEBS Letters. https://doi.org/10.1016/s0014-5793(99)00696-1
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