The DOI matches its Crossref record; 9 of the 9 references it cites were found in a registry or an index.
Adverse effects of the classic antioxidant uric acid in adipocytes: NADPH oxidase-mediated oxidative/nitrosative stress
Abstract
Uric acid is considered a major antioxidant in human blood that may protect against aging and oxidative stress. Despite its proposed protective properties, elevated levels of uric acid are commonly associated with increased risk for cardiovascular disease and mortality. Furthermore, recent experimental studies suggest that uric acid may have a causal role in hypertension and metabolic syndrome. All these conditions are thought to be mediated by oxidative stress. In this study we demonstrate that differentiation of cultured mouse adipocytes is associated with increased production of reactive oxygen species (ROS) and uptake of uric acid. Soluble uric acid stimulated an increase in NADPH oxidase activity and ROS production in mature adipocytes but not in preadipocytes. The stimulation of NADPH oxidase-dependent ROS by uric acid resulted in activation of MAP kinases p38 and ERK1/2, a decrease in nitric oxide bioavailability, and an increase in protein nitrosylation and lipid oxidation. Collectively, our results suggest that hyperuricemia induces redox-dependent signaling and oxidative stress in adipocytes. Since oxidative stress in the adipose tissue has recently been recognized as a major cause of insulin resistance and cardiovascular disease, hyperuricemia-induced alterations in oxidative homeostasis in the adipose tissue might play an important role in these derangements.
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Cites
The NOX Family of ROS-Generating NADPH Oxidases: Physiology and Pathophysiology
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Increased oxidative stress in obesity and its impact on metabolic syndrome
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Inflammation, stress, and diabetes
Wellen, K. E., & Hotamisligil, G. S. (2005) · Journal of Clinical Investigation · doi:10.1172/jci25102 · Cited by 4,143
NOX enzymes and the biology of reactive oxygen
Lambeth, J. D. (2004) · Nature Reviews Immunology · doi:10.1038/nri1312 · Cited by 3,125
Reactive oxygen species have a causal role in multiple forms of insulin resistance
Houstis, N., Rosen, E. D., & Lander, E. S. (2006) · Nature · doi:10.1038/nature04634 · Cited by 2,525 · cited 1×
“Albeit there is tremendous complexity of these disorders, an unambiguous common pathogenetic feature for all of them is, paradoxically, an involvement of oxidative stress and oxidative modifications of proteins and lipids as well as redox-dependent low-grade inflammation (5, 13, 18, 53, 57).”
BackgroundFunctional analysis of Nox4 reveals unique characteristics compared to other NADPH oxidases
Martyn, K. D., Frederick, L. M., von Loehneysen, K., Dinauer, M. C., & Knaus, U. G. (2006) · Cellular Signalling · doi:10.1016/j.cellsig.2005.03.023 · Cited by 783
Oxidative stress and diabetic cardiovascular complications
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Nakagawa, T., Tuttle, K. R., Short, R. A., & Johnson, R. J. (2005) · Nature Clinical Practice Nephrology · doi:10.1038/ncpneph0019 · Cited by 382
Self-citationTaillé, C., El-Benna, J., Lanone, S., Boczkowski, J., & Motterlini, R. (2005) · Journal of Biological Chemistry · doi:10.1074/jbc.m503512200 · Cited by 246 · cited 1×
“NADPH oxidase activity was measured using the lucigenin-enhanced chemiluminescence method in crude cell homogenates and microsomal membrane fractions by following previously described procedures with minor modifications (15, 54).”
Method
Cited by
Johnson, R. J., Segal, M. S., Sautin, Y., Nakagawa, T., Feig, D. I., Kang, D.-H., Gersch, M. S., Benner, S., & Sánchez-Lozada, L. G. (2007) · The American Journal of Clinical Nutrition · doi:10.1093/ajcn/86.4.899 · Cited by 1,075
Self-citationFeig, D. I., Soletsky, B., & Johnson, R. J. (2008) · JAMA · doi:10.1001/jama.300.8.924 · Cited by 914
Self-citationSugar, Uric Acid, and the Etiology of Diabetes and Obesity
Johnson, R. J., Nakagawa, T., Sanchez-Lozada, L. G., Shafiu, M., Sundaram, S., Le, M., Ishimoto, T., Sautin, Y. Y., & Lanaspa, M. A. (2013) · Diabetes · doi:10.2337/db12-1814 · Cited by 760
Self-citationUric Acid Induces Hepatic Steatosis by Generation of Mitochondrial Oxidative Stress
Lanaspa, M. A., Sanchez-Lozada, L. G., Choi, Y.-J., Cicerchi, C., Kanbay, M., Roncal-Jimenez, C. A., Ishimoto, T., Li, N., Marek, G., Duranay, M., et al. (2012) · Journal of Biological Chemistry · doi:10.1074/jbc.m112.399899 · Cited by 710
Self-citationYu, M.-A., Sánchez-Lozada, L. G., Johnson, R. J., & Kang, D.-H. (2010) · Journal of Hypertension · doi:10.1097/hjh.0b013e328337da1d · Cited by 626
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Metadata
- Authors
- Sautin, Y. Y., Nakagawa, T., Zharikov, S., & Johnson, R. J.
- Venue
- American Journal of Physiology-Cell Physiology
- Publisher
- American Physiological Society
- Published
- 2007-08
- Volume
- 293
- Issue
- 2
- Pages
- C584-C596
- ISSN
- 0363-6143 (ISSN-L)
- 1522-1563
- OpenAlex
- W2171576765
- Cited by
- 787
- References
- 9
- FWCI
- 17.87
- Type
- journal article
- Language
- en
- Open access
- closed
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- Sautin, Y. Y., Nakagawa, T., Zharikov, S., & Johnson, R. J. (2007). Adverse effects of the classic antioxidant uric acid in adipocytes: NADPH oxidase-mediated oxidative/nitrosative stress. American Journal of Physiology-Cell Physiology. https://doi.org/10.1152/ajpcell.00600.2006
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