CoChat
Sign Up
Cited by 100Heat shock proteins researchBarrier Structure and Function StudiesGastrointestinal motility and disorders

Heat-shock protein 72 protects against oxidant-induced injury of barrier function of human colonic epithelial Caco2/bbe cells

Open access
Musch, Mark W., Sugi, Kazunori, Straus, David, Chang, Eugene B.·1999·Gastroenterology
DOI 10.1016/s0016-5085(99)70557-3
Open this paper in CoChat — free accountOpen PDF

Abstract

Background & aims Barrier function of the inflamed intestinal mucosa can be compromised by reactive oxygen metabolites that increase mucosal permeability and disrupt the actin cytoskeleton, the integrity of which is important for maintaining tight epithelial junctions. Because heat-shock protein 72 (hsp72) protects intestinal epithelial cells against injury, we determined whether resistance of Caco2/bbe (C2) intestinal monolayer barrier function was related to their high endogenous hsp72 expression. Methods hsp72 anti-sense (C2/AS) and vector-only transfected C2 (C2/CEP4) clones, lines that exhibit low and high hsp72 expression, respectively, were studied. Permeability was assessed by measuring electrical resistance and mannitol fluxes and actin organization by confocal fluorescein isothiocyanate-phalloidin analysis. Results Basal transepithelial electrical resistance (TER) and mannitol fluxes were not significantly different between groups. However, the oxidant monochloramine rapidly decreased TER and increased mannitol permeability of C2/AS monolayers compared with C2/CEP4 (50% effective doses at 30 minutes were 0.53 +/- 0.11 and 2.06 +/- 0.34 mmol/L, respectively). Associated with these changes, decreased cell viability, dissociation and aggregation of perijunctional and stress actin filaments, loss of cell height, and increased intercellular separation were observed only in C2/AS cells treated with monochloramine. Conclusions hsp72 protects intestinal epithelial barrier function against oxidant-induced stress, in part, by protecting the integrity of the actin cytoskeleton.

Integrity signals

Registry

Crossref · 10.1016/s0016-5085(99)70557-3

Cites

23 of 23 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • Chloramines are membrane penetrating oxidants and react rapidly with sulphhydryl groups of proteins in the cytoskeleton and the extracellular matrix, causing disruption of tight junctions and an almost immediate increase in epithelial permeability.

    “Chloramines are membrane penetrating oxidants and react rapidly with sulphhydryl groups of proteins in the cytoskeleton and the extracellular matrix causing disruption of tight junctions and an almost immediate increase in epithelial permeability, as shown in in vitro studies [ (Tatsumi and Fliss 1994;Nakamura et al. 1995;Musch et al. 1999), as cited by Carbonnelle et al. (2002)].”

    From Inorganic chloramines: a critical review of the toxicological and epidemiological evidence as a basis for occupational exposure limit setting (2020) · cites this paper as Musch et al. 1999

Cites

Flagged first, then by citations

1 of the 24 references couldn’t be matched to a known paper.

Cited by

Sorted by citations

Related

Authors
Musch, M. W., Sugi, K., Straus, D., & Chang, E. B.
Affiliations
University of Chicago (US)
ORCID
Venue
Gastroenterology
Publisher
Elsevier BV
Published
1999-07
Volume
117
Issue
1
Pages
115-122
ISSN
0016-5085 (ISSN-L)
DOI
10.1016/s0016-5085(99)70557-3
OpenAlex
W1997149916
Cited by
100
References
24
Citations by year
2025: 1 · 2024: 4 · 2023: 2 · 2022: 1 · 2020: 2 · 2019: 1 · 2018: 1 · 2017: 3 · 2016: 3 · 2015: 5 · 2014: 3 · 2013: 6 · 2012: 4
FWCI
1.50
Type
journal article
Language
en
Open access
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Gastroenterology (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • University of Chicago (CA-14599)
  • National Institutes of Health
  • National Cancer Institute (CA 14599, P30CA014599)
  • National Institute of Diabetes and Digestive and Kidney Diseases (DK-47722, DK42086, P30DK042086)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Heat shock proteins research
  • Life Sciences › Neuroscience › Neurology › Barrier Structure and Function Studies
  • Health Sciences › Medicine › Gastroenterology › Gastrointestinal motility and disorders
Keywords
HSP72, intestinal epithelial barrier function, monochloramine, reactive oxygen species, actin cytoskeleton, tight junctions, transepithelial electrical resistance, mannitol permeability, intestinal permeability
MeSH terms
Actins, Cell Line, Cell Survival, Chloramines, Colon, Cytoskeleton, Heat-Shock Proteins, Humans, Intestinal Mucosa, Mannitol, Permeability, Oligonucleotides, Antisense, Oxidants, Electric Impedance, Caco-2 Cells, HSP72 Heat-Shock Proteins

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review