Dietary determinants of serum paraoxonase activity in healthy humans
Abstract
The associations between habitual diet and a variety of markers of lipid peroxidation or oxidative stress in a group of 95 healthy comparatively young Finnish volunteers (24 male and 71 females) were investigated. The habitual diet of the subjects was evaluated with a 3-day food record. The following biochemical parameters related to lipid peroxidation or oxidative stress were measured: lagtime of Cu2+ induced LDL oxidation in vitro, lipid hydroperoxides and Schiff bases produced during the LDL oxidation test, malondialdehyde measured as thiobarbituric acid-reactive substances from native LDL and Cu2+ oxidized LDL, serum paraoxonase (PON) activity. Serum PON activity showed most constantly associations with habitual diet. PON activity correlated negatively (r=-0.31 to -0.37) with intake of vegetables, total and water-soluble fiber, as well as intake of beta-carotene. Highly significant difference (P=0.005) in PON activity between lowest (<135 g/day) and highest (>256 g/day) vegetable intake quartiles was found. Malondialdehyde levels showed conflicting associations with diet. The results suggest that the significantly lower PON activity associated with high vegetable intake needs to be studied further.
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Serum PON1 activity was measured using a spectroscopy method and Paraoxon (diethyl-p-nitrophenyl phosphate; Sigma) as the substrate.
“Serum PON1 activity was measured using spectroscopy method and Paraoxon (diethyl-p-nitrophenyl phosphate; Sigma) as the substrate92.”
From Prolonged sleep restriction induces changes in pathways involved in cholesterol metabolism and inflammatory responses (2016) · cites this paper as [92]
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- Authors
- Kleemola, P., Freese, R., Jauhiainen, M., Pahlman, R., Alfthan, G., & Mutanen, M.
- Affiliations
- University of Helsinki (FI)
- Corresponding author
- Mutanen, Marja
- Venue
- Atherosclerosis
- Publisher
- Elsevier BV
- Published
- 2002-02
- Volume
- 160
- Issue
- 2
- Pages
- 425-432
- ISSN
- 0021-9150 (ISSN-L)
- OpenAlex
- W2127387592
- Cited by
- 101
- References
- 47
- Citations by year
- 2025: 2 · 2024: 2 · 2023: 2 · 2022: 4 · 2021: 3 · 2020: 2 · 2019: 4 · 2017: 3 · 2016: 5 · 2015: 6 · 2014: 7 · 2013: 5 · 2012: 3
- FWCI
- 5.03
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Atherosclerosis (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Clinical Biochemistry › Paraoxonase enzyme and polymorphisms
- Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Nutritional Studies and Diet
- Keywords
- serum paraoxonase activity, dietary factors, lipid peroxidation, oxidative stress, vegetable consumption, dietary fiber, beta-carotene, malondialdehyde, LDL oxidation, lipid hydroperoxides, Schiff base, healthy young adults
- MeSH terms
- Adult, Diet, Esterases, Female, Humans, Lipoproteins, LDL, Male, Malondialdehyde, Middle Aged, Oxidation-Reduction, Lipid Peroxidation, Oxidative Stress, Aryldialkylphosphatase
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