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Cited by 85Atherosclerosis and Cardiovascular DiseasesCholesterol and Lipid MetabolismCerebrovascular and Carotid Artery Diseases

Costimulatory molecules in human atherosclerotic plaques: an indication of antigen specific T lymphocyte activation

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de Boer, Onno J, Hirsch, Floris, van der Wal, Allard C, van der Loos, Chris M, Das, Pranab K, Becker, Anton E·1997·Atherosclerosis
DOI 10.1016/s0021-9150(97)00135-4
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Abstract

Atherosclerotic plaques contain inflammation, composed largely of macrophages and lymphocytes. A proportion of lymphocytes shows signs of activation, but the question arises whether they are activated in an antigen specific way. The expression of costimulatory molecules-receptors that provide accessory signals during antigen-specific activation is a prerequisite for such a condition. This aspect of inflammation in atherosclerotic lesions has not been investigated. Human arterial segments with diffuse intimal thickening, fatty streaks and atherosclerotic plaques were studied with immuno-single and double staining methods. Macrophages and T lymphocytes were stained with CD68 and CD3, respectively, and pan-B cell markers CD19 and CD22 were also used. Costimulatory molecules B7-1 and B7-2, together with their common ligand CD28, and CD27 with its ligand CD70, were stained with specific monoclonal antibodies. The results show that most T lymphocytes were CD27 positive and that only a subpopulation of these (5-15%) was positive also for B7-1, CD28 and CD70. Macrophages expressed B7-1, B7-2, CD28 and CD70, while macrophages positive for CD28 and CD70 have not been reported yet. The expression of costimulatory molecules was most pronounced in the superficial layers at the fibrous cap, but decreased towards the lipid core. This study shows, therefore, that atherosclerotic plaques provide costimulatory signals generally accepted as a prerequisite for adequate T cell stimulation. In addition, this study reveals that only approximately 5-15% of the lymphocytes appears actively involved in the inflammatory reaction.

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  • Immunohistochemical analysis of atherosclerotic plaques from human arteries, including the descending aorta, revealed CD80 expression in macrophages and a small subset of T cells predominantly localized to the fibrous cap, linking CD80 expression to the inflammatory processes underlying plaque development.

    “Immunohistochemical analysis of atherosclerotic plaques from human arteries, including the descending aorta, revealed CD80 expression in macrophages and a small subset of T cells, predominantly localized to the fibrous cap, thereby linking CD80 expression to the inflammatory processes underlying plaque development 32 .”

    From Connecting proteomics and genomics to identify causal biomarkers specific for aortic valve stenosis (2025) · cites this paper as [32]

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Research works printed but not linked: 4

  1. Stemme S, Hansson GK. Immune mechanisms in atherosclero- sis. Coron Artery Dis 1994;5:216 -22.
  2. Harding FA, McArthur JG, Gross JA, Raulet DH, Allison JP. CD28 mediated signaling costimulates murine T cells and pre- vent induction of anergy in T cell clones. Nature 1992;356:455- 9.
  3. Van der Loos CM, Das PK, van den Oord JJ, Houthoff HJ. Multiple immunoenzyme staining techniques. Use of fluores- ceinated, biotinylated and unlabeled staining techniques. J Im- munol Methods 1989;117:45-52.
  4. Azuma M, Yssel H, Phillips JH, Spits H, Lanier LL. Func- tional expression of B7/BB1 on activated T lymphozytes. J Exp Med 1993;177:845 -50.

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Authors
de Boer, O. J., Hirsch, F., van der Wal, A. C., van der Loos, C. M., Das, P. K., & Becker, A. E.
Affiliations
Amsterdam UMC Location University of Amsterdam (NL)
Corresponding author
Becker, Anton E
ORCID
Das, Pranab K
Venue
Atherosclerosis
Publisher
Elsevier BV
Published
1997-09
Volume
133
Issue
2
Pages
227-234
ISSN
0021-9150 (ISSN-L)
DOI
10.1016/s0021-9150(97)00135-4
OpenAlex
W1980750537
Cited by
85
References
37
Citations by year
2026: 1 · 2025: 2 · 2024: 2 · 2022: 3 · 2021: 2 · 2020: 2 · 2019: 3 · 2018: 4 · 2017: 5 · 2016: 1 · 2015: 2 · 2014: 3 · 2013: 4 · 2012: 2
FWCI
1.16
Type
journal article
Language
en
Open access
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Atherosclerosis (publishedVersion)
  • PubMed (publishedVersion)
  • UvA-DARE (University of Amsterdam) (publishedVersion)
  • Data Archiving and Networked Services (DANS) (submittedVersion)
  • Pure Amsterdam UMC (submittedVersion)
  • Data Archiving and Networked Services (DANS) (submittedVersion, other-oa)
Topics
  • Life Sciences › Immunology and Microbiology › Immunology › Atherosclerosis and Cardiovascular Diseases
  • Health Sciences › Medicine › Surgery › Cholesterol and Lipid Metabolism
  • Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Cerebrovascular and Carotid Artery Diseases
Keywords
costimulatory molecules, atherosclerotic plaque, T cell activation, CD80, CD86, CD28, CD27, CD70, macrophages, T cell subsets, fibrous cap, lipid core
MeSH terms
CD68 Molecule, Aged, Aged, 80 and over, Epitopes, Antigens, Differentiation, B-Lymphocyte, Arteries, Arteriosclerosis, Endothelium, Vascular, Humans, Immunohistochemistry, Lymphocyte Activation, Macrophages, Membrane Glycoproteins, Membrane Proteins, Middle Aged, T-Lymphocytes, Antigens, Differentiation, Myelomonocytic, Antigens, CD, Cell Adhesion Molecules, CD3 Complex, CD28 Antigens, B7-1 Antigen, Tumor Necrosis Factor Receptor Superfamily, Member 7, Antigens, CD19, Lectins, Sialic Acid Binding Ig-like Lectin 2, B7-2 Antigen, CD27 Ligand

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