Cited by 173HIV Research and TreatmentDNA and Nucleic Acid ChemistryGenomics and Chromatin Dynamics

The influence of DNA and nucleosome structure on integration events directed by HIV integrase

Open access
Pruss, D., Reeves, R., Bushman, F.D., Wolffe, A.P.·1994·Journal of Biological Chemistry

Abstract

DNA copies of the human immunodeficiency virus (HIV) genome integrate nonrandomly into the chromosomal DNA of the host cell. In this report, we investigate the molecular basis of this selectivity using the virus-encoded HIV integrase to direct integration of a synthetic HIV long terminal repeat substrate into either DNA molecules of known structure or previously defined nucleosomal complexes. We find that the structure of the target greatly influences the site of integration, and, moreover, DNA curvature, flexibility, and rigidity in solution all influence the frequency of integration. Importantly, for DNA with all of these properties, the distortion of the double helix directed by association with the histone proteins promotes the integration reaction and alters the distribution of sites that are selected for integration. We suggest that both intrinsic DNA structure and the folding of DNA into chromosomal structures will exert a major influence on target site selection for integration of the viral genome.

Integrity signals

Registry

Crossref · 10.1016/s0021-9258(17)31494-1

Cites

63 of 63 references verified0 citation errors · 0 not checkable

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  • PIC or intasome have greater inclination to integrate into bent target DNA wrapped with histones or transcription factors compared to naked DNA.

    “It has been observed that PIC or the intasome have greater inclination to integrate into bent target DNA that is wrapped with histones or transcription factors in comparison to the naked DNA (Müller and Varmus 1994, Pruss et al. 1994, Maskell et al. 2015, Matysiak et al. 2017).”

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Authors
Pruss, D., Reeves, R., Bushman, F. D., & Wolffe, A. P.
Affiliations
National Institutes of Health (US)
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
1994-10
Volume
269
Issue
40
Pages
25031-25041
ISSN
0021-9258 (ISSN-L)
OpenAlex
W1569658633
Cited by
173
References
63
Citations by year
2025: 2 · 2024: 2 · 2023: 2 · 2022: 2 · 2021: 4 · 2020: 3 · 2019: 5 · 2018: 3 · 2017: 6 · 2016: 3 · 2015: 7 · 2014: 8 · 2013: 2 · 2012: 2
FWCI
2.56
Type
journal article
Language
en
Open access
Locations
  • Journal of Biological Chemistry (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Immunology and Microbiology › Virology › HIV Research and Treatment
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
Keywords
HIV integrase, viral DNA integration, target site selection, nucleosome structure, DNA curvature, DNA flexibility, histone proteins, long terminal repeat, chromatin
MeSH terms
Base Sequence, DNA, DNA Nucleotidyltransferases, HIV, Molecular Sequence Data, Nucleosomes, Virus Integration, Integrases

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