Central Core Domain of Retroviral Integrase Is Responsible for Target Site Selection
Open accessAbstract
Integration of retroviral DNA can occur into many sites on target DNA with a wide variation in preference. One factor known to affect target site selection is integrase, the viral protein required for the integration reaction. In this study, assays that measure the distribution and frequency of retroviral DNA integration showed that purified integrases of human immunodeficiency virus type 1 (HIV-1) and feline immunodeficiency virus (FIV) had different patterns of target site usage. The integrase domain involved in target site selection was mapped by analyzing the integration pattern of chimeric proteins formed between HIV-1 and FIV integrases and of deletion variants of the two wild-type integrases. The results indicate that the domain responsible for target site selection resides in the central core region of integrase.
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The IN core domain has D64, D116, and E152, collectively known as a catalytic triad.
“The IN core domain has D64, D116, and E152, which are collectively known as a catalytic triad [28].”
From Non-Integrating Lentiviral Vectors in Clinical Applications: A Glance Through (2022) · cites this paper as [28]
Cites
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Non-Integrating Lentiviral Vectors in Clinical Applications: A Glance Through
Gurumoorthy, N., Nordin, F., Tye, G. J., Wan Kamarul Zaman, W. S., & Ng, M. H. (2022) · Biomedicines · doi:10.3390/biomedicines10010107 · Cited by 69 · see citing works
Tekeste, S. S., Wilkinson, T. A., Weiner, E. M., Xu, X., Miller, J. T., Le Grice, S. F. J., Clubb, R. T., & Chow, S. A. (2015) · Journal of Virology · doi:10.1128/jvi.01471-15 · Cited by 51 · see citing works
Self-citation
- Authors
- hio Shibagaki, Y., & Chow, S. A.
- Affiliations
- University of California, Los Angeles (US)
- Corresponding author
- Chow, Samson A.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1997-03
- Volume
- 272
- Issue
- 13
- Pages
- 8361-8369
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2028692066
- Cited by
- 73
- References
- 3
- Citations by year
- 2023: 1 · 2022: 3 · 2016: 1 · 2015: 3 · 2014: 1 · 2013: 2
- FWCI
- 1.63
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health
- National Cancer Institute (R01CA068859, R01 CA68859)
- Topics
- Life Sciences › Immunology and Microbiology › Virology › HIV Research and Treatment
- Health Sciences › Medicine › Infectious Diseases › HIV/AIDS drug development and treatment
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Virus-based gene therapy research
- Keywords
- retroviral integrase, target site selection, HIV-1 integrase, DNA integration
- MeSH terms
- Amino Acid Sequence, Binding Sites, DNA, Electrophoresis, Polyacrylamide Gel, Molecular Sequence Data, Recombinant Fusion Proteins, HIV-1, Immunodeficiency Virus, Feline, Polymerase Chain Reaction, Sequence Alignment, Integrases
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