The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.
A “slow” homotetrameric kinesin-related motor protein purified from Drosophila embryos.
Open accessAbstract
Pan-kinesin peptide antibodies (Cole, D. G., Cande, W. Z., Baskin, R. J., Skoufias, D. A., Hogan, C. J., and Scholey, J. M. (1992) J. Cell Sci. 101, 291-301; Sawin, K. E., Mitchinson, T. J., and Wordeman, L. G. (1992) J. Cell Sci. 101, 303-313) were used to identify and isolate kinesin-related proteins (KRPs) from Drosophila melanogaster embryonic cytosol. These KRPs cosedimented with microtubules (MTs) polymerized from cytosol treated with AMP-PNP (adenyl-5'-yl imidodiphosphate), and one of them, KRP130, was further purified from ATP eluates of the embryonic MTs. Purified KRP130 behaves as a homotetrameric complex composed of four 130-kDa polypeptide subunits which displays a "slow" plus-end directed motor activity capable of moving single MTs at 0.04 +/- 0.01 microns/s. The 130-kDa subunit of KRP130 was tested for reactivity with monoclonal and polyclonal antibodies that are specific for various members of the kinesin superfamily. Results indicate that the KRP130 subunit is related to Xenopus Eg5 (Sawin, K. E., Le Guellec, K. L., Philippe, M., Mitchinson, T. J. (1992) Nature 359, 540-543), a member of the BimC subfamily of kinesins. Therefore, KRP130 appears to be the first Drosophila KRP, and the first member of the BimC subfamily in any organism, to be purified from native tissue as a multimeric motor complex.
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Cites
Identification of a novel force-generating protein, kinesin, involved in microtubule-based motility
VALE, R., REESE, T., & SHEETZ, M. (1985) · Cell · doi:10.1016/s0092-8674(85)80099-4 · Cited by 1,993
Hirokawa, N., Pfister, K. K., Yorifuji, H., Wagner, M. C., Brady, S. T., & Bloom, G. S. (1989) · Cell · doi:10.1016/0092-8674(89)90691-0 · Cited by 477
The carboxyl-terminal domain of kinesin heavy chain is important for membrane binding
Skoufias, D. A., Cole, D. G., Wedaman, K. P., & Scholey, J. M. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)42281-2 · Cited by 110
Self-citationThe Drosophila kinesin light chain. Primary structure and interaction with kinesin heavy chain
Gauger, A. K., & Goldstein, L. S. (1993) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)38698-3 · Cited by 105
Characterization of α2β2 and α2 forms of kinesin
Hackney, D. D., Levitt, J. D., & Wagner, D. D. (1991) · Biochemical and Biophysical Research Communications · doi:10.1016/0006-291x(91)91490-4 · Cited by 80
Cited by
Analysis of the kinesin superfamily: insights into structure and function
Miki, H., Okada, Y., & Hirokawa, N. (2005) · Trends in Cell Biology · doi:10.1016/j.tcb.2005.07.006 · Cited by 719
Sharp, D. J., Rogers, G. C., & Scholey, J. M. (2000) · Nature · doi:10.1038/35024000 · Cited by 592
Self-citationThe design plan of kinesin motors
Vale, R. D., & Fletterick, R. J. (1997) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev.cellbio.13.1.745 · Cited by 474
Kashlna, A. S., Baskin, R. J., Cole, D. G., Wedaman, K. P., Saxton, W. M., & Scholey, J. M. (1996) · Nature · doi:10.1038/379270a0 · Cited by 352
Self-citationSharp, D. J., McDonald, K. L., Brown, H. M., Matthies, H. J., Walczak, C., Vale, R. D., Mitchison, T. J., & Scholey, J. M. (1999) · The Journal of Cell Biology · doi:10.1083/jcb.144.1.125 · Cited by 313
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Metadata
- Authors
- Cole, D. G., Saxton, W. M., Sheehan, K. B., & Scholey, J. M.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1994-09
- Volume
- 269
- Issue
- 37
- Pages
- 22913-22916
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1507086201
- Cited by
- 181
- References
- 5
- FWCI
- 3.21
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Cole, D. G., Saxton, W. M., Sheehan, K. B., & Scholey, J. M. (1994). A “slow” homotetrameric kinesin-related motor protein purified from Drosophila embryos. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(17)31593-4
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