The DOI matches its Crossref record; 9 of the 9 references it cites were found in a registry or an index.
Why does Coomassie Brilliant Blue R interact differently with different proteins? A partial answer.
Open accessAbstract
Dimethyl sulfoxide was found to be effective for extraction of Coomassie Brilliant Blue R-250 (Coomassie R) from stained proteins on polyacrylamide gel slices. A good correlation was found between the ability of different proteins to bind Coomassie R and their capacity for interaction with Coomassie Brilliant Blue G-250 (Coomassie G) in solution. Scatchard analysis showed that the number of Coomassie R ligands bound to each protein molecule is approximately proportional to the number of positive charges on the protein, about 1.5-3 dye molecules/charge.
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Cites
Bradford, M. M. (1976) · Analytical Biochemistry · doi:10.1016/0003-2697(76)90527-3 · Cited by 255,302
Weber, K., & Osborn, M. (1969) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)94333-4 · Cited by 20,298
Atlas of protein sequence and structure
Chang, M.-Y., Dayhoff, M. O., Eck, R. V., & Sochard, M. R. (1965) · NASA Technical Reports Server (NASA) · Cited by 4,383
Read, S. M., & Northcote, D. H. (1981) · Analytical Biochemistry · doi:10.1016/0003-2697(81)90321-3 · Cited by 1,421
Two new staining procedures for quantitative estimation of proteins on electrophoretic strips
De St. Groth, S. F., Webster, R. G., & Datyner, A. (1963) · Biochimica et Biophysica Acta · doi:10.1016/0006-3002(63)91092-8 · Cited by 616
The Binding of Divers Detergent Anions to Bovine Serum Albumin*
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Isoelectric focusing of peptides
Righetti, P. G., & Chillemi, F. (1978) · Journal of Chromatography A · doi:10.1016/s0021-9673(00)92339-2 · Cited by 126
Assay for protein by dye binding
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Cited by
Protein tagging and detection with engineered self-assembling fragments of green fluorescent protein
Cabantous, S., Terwilliger, T. C., & Waldo, G. S. (2005) · Nature Biotechnology · doi:10.1038/nbt1044 · Cited by 938
Peng, J., Kim, M. J., Cheng, D., Duong, D. M., Gygi, S. P., & Sheng, M. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m400103200 · Cited by 473
Sawant, S., & Girard, A. L. (2025) · Journal of Agricultural and Food Chemistry · doi:10.1021/acs.jafc.5c07627 · Cited by 14
Metadata
- Authors
- Tal, M., Silberstein, A., & Nusser, E.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1985-08
- Volume
- 260
- Issue
- 18
- Pages
- 9976-9980
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1518917058
- Cited by
- 274
- References
- 9
- FWCI
- 5.40
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Tal, M., Silberstein, A., & Nusser, E. (1985). Why does Coomassie Brilliant Blue R interact differently with different proteins? A partial answer. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(17)39198-6
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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