Semiquantitative Proteomic Analysis of Rat Forebrain Postsynaptic Density Fractions by Mass Spectrometry
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The postsynaptic density (PSD) of central excitatory synapses plays a key role in postsynaptic signal transduction and contains a high concentration of glutamate receptors and associated scaffold and signaling proteins. We report here a comprehensive analysis of purified PSD fractions by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). We identified 374 different proteins that copurified with the PSD structure and discovered thirteen phosphorylated sites from eight proteins. These proteins were classified into numerous functional groups, implying that the signaling pathways in the PSD are complex and diverse. Furthermore, using quantitative mass spectrometry, we measured the molar concentration and relative stoichiometries of a number of glutamate receptor subunits and scaffold proteins in the postsynaptic density. Thus this proteomic study reveals crucial information about molecular abundance as well as molecular diversity in the PSD, and provides a basis for further studies on the molecular mechanisms of synaptic function and plasticity.
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Ral is expressed in the nervous system.
“Like the exocyst, Ral is expressed in the nervous system (Ngsee et al, 1991;Huber et al, 1994;Peng et al, 2004;Han et al, 2009).”
From Ral mediates activity‐dependent growth of postsynaptic membranes via recruitment of the exocyst (2013) · cites this paper as Peng et al, 2004
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- Authors
- Peng, J., Kim, M. J., Cheng, D., Duong, D. M., Gygi, S. P., & Sheng, M.
- Affiliations
- Harvard University (US)
- Howard Hughes Medical Institute (US)
- Emory University (US)
- Corresponding author
- Peng, Junmin
- Sheng, Morgan
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2004-05
- Volume
- 279
- Issue
- 20
- Pages
- 21003-21011
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2044336832
- Cited by
- 473
- References
- 4
- Citations by year
- 2026: 4 · 2025: 10 · 2024: 7 · 2023: 7 · 2022: 9 · 2021: 10 · 2020: 19 · 2019: 22 · 2018: 20 · 2017: 20 · 2016: 16 · 2015: 19 · 2014: 21 · 2013: 27 · 2012: 34
- FWCI
- 17.76
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Physical Sciences › Chemistry › Spectroscopy › Mass Spectrometry Techniques and Applications
- Keywords
- postsynaptic density, forebrain, glutamate receptors, synaptic proteins, protein phosphorylation, protein stoichiometry, synaptic signaling, excitatory synapses, synaptic plasticity, scaffold proteins
- MeSH terms
- Amino Acid Sequence, Animals, Chromatography, Liquid, Molecular Sequence Data, Nerve Tissue Proteins, Peptide Fragments, Mass Spectrometry, Synapses, Prosencephalon, Proteome, Rats
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