The DOI matches its Crossref record.
Specificity of binding of a strain of uropathogenic Escherichia coli to Gal alpha 1—-4Gal-containing glycosphingolipids.
Open accessAbstract
A strain of Escherichia coli originally isolated from urine of a patient with acute pyelonephritis was studied in detail for binding to glycosphingolipids. Bacteria labeled metabolically with [14C]glucose were layered over a glycolipid chromatogram and bound bacteria were detected by autoradiography. The detection was down to a few ng of glycolipid (pmol level) under these assay conditions. At a test level of 500 ng all glycolipids (more than a dozen molecular species analyzed) with Gal alpha 1----4Gal as an internal or terminal part bound the bacteria strongly while glycolipids known to lack this sequence were negative. Conformational analysis using hard sphere calculations including the exo-anomeric effect showed a bend in the saccharide chain at this disaccharide with a largely hydrophobic surface of the convex side, probably being part of the binding epitope. Mixtures of glycolipids isolated from a human ureter scraping and from urinary sediments bound bacteria in the 2- to 7-sugar interval. Thus, this infectious strain of E. coli recognizes glycolipids being present in epithelial cells lining the urinary tract.
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Citations
Cited by
Identification of the carbohydrate receptor for Shiga toxin produced by Shigella dysenteriae type 1.
Lindberg, A. A., Brown, J. E., Strömberg, N., Westling-Ryd, M., Schultz, J. E., & Karlsson, K. A. (1987) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)75706-8 · Cited by 451
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Hultgren, S. J., Abraham, S., Caparon, M., Falk, P., Geme, J. W. S., & Normark, S. (1993) · Cell · doi:10.1016/0092-8674(93)90269-v · Cited by 409
Leffler, H., & Barondes, S. H. (1986) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)67499-x · Cited by 296
Self-citationKiarash, A., Boyd, B., & Lingwood, C. A. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)78102-2 · Cited by 206
Westerlund, B., van Die, I., Kramer, C., Kuusela, P., Holthöfer, H., Tarkkanen, A. ‐., Virkola, R., Riegman, N., Bergmans, H., Hoekstra, W., et al. (1991) · Molecular Microbiology · doi:10.1111/j.1365-2958.1991.tb01856.x · Cited by 59
Metadata
- Authors
- Bock, K., Breimer, M. E., Brignole, A., Hansson, G. C., Karlsson, K. A., Larson, G., Leffler, H., Samuelsson, B. E., Strömberg, N., & Edén, C. S.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1985-07
- Volume
- 260
- Issue
- 14
- Pages
- 8545-8551
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1604295453
- Cited by
- 293
- References
- 0
- FWCI
- 14.93
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- CoChat. (2026). Citation check: Specificity of binding of a strain of uropathogenic Escherichia coli to Gal alpha 1—-4Gal-containing glycosphingolipids. https://app.cochat.ai/papers/10.1016/s0021-9258(17)39507-8
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- Bock, K., Breimer, M. E., Brignole, A., Hansson, G. C., Karlsson, K. A., Larson, G., Leffler, H., Samuelsson, B. E., Strömberg, N., & Edén, C. S. (1985). Specificity of binding of a strain of uropathogenic Escherichia coli to Gal alpha 1—-4Gal-containing glycosphingolipids. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(17)39507-8
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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