The DOI matches its Crossref record.
Bacteriophage P1 site-specific recombination. Purification and properties of the Cre recombinase protein.
Open accessAbstract
Bacteriophage P1 encodes a site-specific recombination system that consists of a site (loxP) at which recombination occurs and a gene, cre, whose protein product is essential for recombination. The loxP-Cre recombination event can be studied in greater detail by the use of an in vitro system that efficiently carries out recombination between two loxP sites. This paper presents a purification and characterization of the Cre protein (Mr = 35,000), which is the only protein required for the in vitro reaction. No high energy cofactors are needed. The purified Cre protein binds to loxP-containing DNA and makes complexes that are resistant to heparin. Cre efficiently converts 70% of the DNA substrate to products and appears to act stoichiometrically. The action of Cre on a loxP2 supercoiled substrate containing two directly repeated loxP sites results in product molecules that are topologically unlinked. Several models to account for the ability of Cre to produce free supercoiled products are discussed.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
Site-specific DNA recombination in mammalian cells by the Cre recombinase of bacteriophage P1.
Sauer, B., & Henderson, N. (1988) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.85.14.5166 · Cited by 1,213
Bushman, F. D., & Craigie, R. (1991) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.88.4.1339 · Cited by 410
Mechanism of strand cleavage and exchange in the Cre-lox site-specific recombination system
Hoess, R. H., & Abremski, K. (1985) · Journal of Molecular Biology · doi:10.1016/0022-2836(85)90224-4 · Cited by 224
Self-citationWerneck de Castro, J. P., Fonseca, T. L., Ueta, C. B., McAninch, E. A., Abdalla, S., Wittmann, G., Lechan, R. M., Gereben, B., & Bianco, A. C. (2015) · Journal of Clinical Investigation · doi:10.1172/jci77588 · Cited by 184
Bacteriophage P1 cre gene and its regulatory region
Sternberg, N., Sauer, B., Hoess, R., & Abremski, K. (1986) · Journal of Molecular Biology · doi:10.1016/0022-2836(86)90228-7 · Cited by 171
Self-citationCoordination of hypothalamic and pituitary T3 production regulates TSH expression
Fonseca, T. L., Correa-Medina, M., Campos, M. P. O., Wittmann, G., Werneck-de-Castro, J. P., Arrojo e Drigo, R., Mora-Garzon, M., Ueta, C. B., Caicedo, A., Fekete, C., et al. (2013) · Journal of Clinical Investigation · doi:10.1172/jci61231 · Cited by 155
Robotic cloning and protein production platform of the northeast structural genomics consortium
Acton, T. B., Gunsalus, K. C., Xiao, R., Ma, L. C., Aramini, J., Baran, M. C., Chiang, Y.-W., Climent, T., Cooper, B., Denissova, N. G., et al. (2005) · Methods in Enzymology · doi:10.1016/s0076-6879(05)94008-1 · Cited by 129
Schleidgen, S., Dederer, H.-G., Sgodda, S., Cravcisin, S., Lüneburg, L., Cantz, T., & Heinemann, T. (2020) · BMC Medical Ethics · doi:10.1186/s12910-020-00487-1 · Cited by 43
Construction of a human hTERT RPE-1 cell line with inducible Cre for editing of endogenous genes
Hindul, N. L., Jhita, A., Oprea, D. G., Hussain, T. A., Gonchar, O., Campillo, M. A. M., O'Regan, L., Kanemaki, M. T., Fry, A. M., Hirota, K., et al. (2022) · Biology Open · doi:10.1242/bio.059056 · Cited by 12
Constitutive expression of CX3CR1-BAC-Cre introduces minimal off-target effects in microglia
Mroue-Ruiz, F. H., Desai, B., Garvin, M. M., Shehu, J. H., Kamau, F., Kar, U., & Bolton, J. L. (2026) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2026.153657 · Cited by 3
Metadata
- Authors
- Abremski, K., & Hoess, R.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1984-02
- Volume
- 259
- Issue
- 3
- Pages
- 1509-1514
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1678580492
- Cited by
- 324
- References
- 0
- FWCI
- 16.61
- Type
- journal article
- Language
- en
- Open access
- hybrid
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
