Bacteriophage P1 cre gene and its regulatory region
Abstract
The bacteriophage P1 site-specific recombination system consists of two components, a site, loxP, at which recombination occurs, and a recombinase protein, Cre. In this paper, we present the DNA sequence of the cre structural gene and its upstream regulatory region. Analysis of the sequence indicates: (1) that cre encodes a protein of 343 amino acids; (2) that cre and loxP are separated by a 434 base-pair region that contains a 73 amino acid open reading frame, orf1; and (3) that cre and orf1 are oriented with their amino-terminal ends proximal to loxP. We have identified three promoters that are located upstream of the cre structural gene. Their activities range from 7 to 10% of the activity of the galactose operon promoter. The promoter furthest from cre, pR1, contains two Dam methylation sites (5'-G-A-T-C-3') in its -35 region, and is sensitive to Dam methylation. Its transcription is three- to fourfold higher in a dam- host than it is in a dam+ host. The promoter closest to cre, pR3, signals the production of an RNA transcript that functions inefficiently for Cre protein synthesis because it lacks a ribosome recognition site. None of the three cre promoters is sensitive to proteins expressed by the P1 prophage, including the c1 repressor protein. To assess the role of cre in the P1 life-cycle, we isolated cre mutants and studied their behavior in recA+ and recA- hosts. Those studies indicate that Cre is dispensable for viral vegetative growth and lysogeny in a recA+ host,…
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Cites
Experiments in molecular genetics
Miller, J. H. (1972) · Medical Entomology and Zoology · Cited by 27,157 · see citing works
Vieira, J., & Messing, J. W. (1982) · Gene · doi:10.1016/0378-1119(82)90015-4 · Cited by 6,127 · see citing works
A rapid boiling method for the preparation of bacterial plasmids
Holmes, D. S., & Quigley, M. E. (1981) · Analytical Biochemistry · doi:10.1016/0003-2697(81)90473-5 · Cited by 3,198 · see citing works
REGULATORY SEQUENCES INVOLVED IN THE PROMOTION AND TERMINATION OF RNA TRANSCRIPTION
Rosenberg, M. J., & Court, D. (1979) · Annual Review of Genetics · doi:10.1146/annurev.ge.13.120179.001535 · Cited by 2,726 · see citing works
A rapid method for determining sequences in DNA by primed synthesis with DNA polymerase
Sanger, F., & Coulson, A. R. (1975) · Journal of Molecular Biology · doi:10.1016/0022-2836(75)90213-2 · Cited by 2,709 · see citing works
Compilation and analysis ofEscherichia colipromoter DNA sequences
Hawley, D. K., & McClure, W. R. (1983) · Nucleic Acids Research · doi:10.1093/nar/11.8.2237 · Cited by 2,217 · see citing works
Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells
Dagert, M., & Ehrlich, S. D. (1979) · Gene · doi:10.1016/0378-1119(79)90082-9 · Cited by 1,458 · see citing works
Appleyard, R. K. (1954) · Genetics · doi:10.1093/genetics/39.4.440 · Cited by 961 · see citing works
Bacteriophage P1 site-specific recombination
Sternberg, N. L., & Hamilton, D. L. (1981) · Journal of Molecular Biology · doi:10.1016/0022-2836(81)90375-2 · Cited by 791 · see citing works
Self-citationCharacterization of translational initiation sites inE. coli
Stormo, G. D., Schneider, T. D., & Gold, L. M. (1982) · Nucleic Acids Research · doi:10.1093/nar/10.9.2971 · Cited by 671 · see citing works
A restriction enzyme cleavage map of Tn5 and location of a region encoding neomycin resistance
Jorgensen, R. A., Rothstein, S. J., & Reznikoff, W. S. (1979) · Molecular and General Genetics MGG · doi:10.1007/bf00267254 · Cited by 577 · see citing works
Gene amplification and analysis
Papas, T. S., & Woude, G. F. V. (1986) · OSTI OAI (U.S. Department of Energy Office of Scientific and Technical Information) · Cited by 517 · see citing works
Lin, F., Sperle, K., & Sternberg, N. L. (1984) · Molecular and Cellular Biology · doi:10.1128/mcb.4.6.1020-1034.1984 · Cited by 461 · see citing works
Self-citationFormation, induction, and curing of bacteriophage P1 lysogens
Rosner, J. L. (1972) · Virology · doi:10.1016/0042-6822(72)90152-3 · Cited by 428 · see citing works
Abremski, K., Hoess, R. H., & Sternberg, N. L. (1983) · Cell · doi:10.1016/0092-8674(83)90311-2 · Cited by 333 · see citing works
Self-citationA novel role for site-specific recombination in maintenance of bacterial replicons
Austin, S. J., Ziese, M., & Sternberg, N. L. (1981) · Cell · doi:10.1016/0092-8674(81)90180-x · Cited by 333 · see citing works
Self-citationAbremski, K., & Hoess, R. H. (1984) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)43437-5 · Cited by 329 · see citing works
Self-citationIn vitro packaging of a λ Dam vector containing EcoRI DNA fragments of Escherichia coli and phage P1
Sternberg, N. L., Tiemeier, D. C., & Enquist, L. W. (1977) · Gene · doi:10.1016/0378-1119(77)90049-x · Cited by 318 · see citing works
Self-citationMechanism of strand cleavage and exchange in the Cre-lox site-specific recombination system
Hoess, R. H., & Abremski, K. (1985) · Journal of Molecular Biology · doi:10.1016/0022-2836(85)90224-4 · Cited by 224 · see citing works
Self-citationBacteriophage P1 site-specific recombination
Sternberg, N. L., Hamilton, D. L., & Hoess, R. H. (1981) · Journal of Molecular Biology · doi:10.1016/0022-2836(81)90376-4 · Cited by 152 · see citing works
Self-citationPackaging of the bacteriophage λ chromosome: Effect of chromosome length
Feiss, M. G., Fisher, R. A., Crayton, M. A., & Egner, C. (1977) · Virology · doi:10.1016/0042-6822(77)90425-1 · Cited by 136 · see citing works
Insertion mutations in the dam gene of Escherichia coli K-12
Marinus, M., Carraway, M., Frey, A. Z., Brown, L., & Arraj, J. A. (1983) · Molecular and General Genetics MGG · doi:10.1007/bf00327681 · Cited by 125 · see citing works
Enquist, L. W., & Weisberg, R. A. (1976) · Virology · doi:10.1016/0042-6822(76)90319-6 · Cited by 100 · see citing works
The control of host-induced modification by phage P1
Glover, S. W., Schell, J., Symonds, N., & Stacey, K. A. (1963) · Genetics Research · doi:10.1017/s0016672300003876 · Cited by 72 · see citing works
Clear plaque mutants of phage P1
Scott, J. R. (1970) · Virology · doi:10.1016/0042-6822(70)90054-1 · Cited by 70 · see citing works
Adenine methylation of Okazaki fragments in Escherichia coli
Marinus, M. (1976) · Journal of Bacteriology · doi:10.1128/jb.128.3.853-854.1976 · Cited by 65 · see citing works
A genetic analysis of bacteriophage λ head assembly
Sternberg, N. L. (1976) · Virology · doi:10.1016/0042-6822(76)90382-2 · Cited by 62 · see citing works
Self-citationA characterization of bacteriophage P1 DNA fragments cloned in a λ Vector
Sternberg, N. L. (1979) · Virology · doi:10.1016/0042-6822(79)90179-x · Cited by 61 · see citing works
Self-citationKinetics of methylation in Escherichia coli K-12
Lyons, S. M., & Schendel, P. F. (1984) · Journal of Bacteriology · doi:10.1128/jb.159.1.421-423.1984 · Cited by 55 · see citing works
Packaging of coliphage lambda DNA
Sternberg, N. L., & Weisberg, R. A. (1977) · Journal of Molecular Biology · doi:10.1016/0022-2836(77)90066-3 · Cited by 52 · see citing works
Self-citationTyrosine-incorporating amber suppressors in Escherichia coli K12
Dennert, G., & Henning, U. (1968) · Journal of Molecular Biology · doi:10.1016/0022-2836(68)90300-8 · Cited by 50 · see citing works
Control of circularization of bacteriophage P1 DNA in Escherichia coli
Segev, N., & Cohen, G. (1981) · Virology · doi:10.1016/0042-6822(81)90215-4 · Cited by 29 · see citing works
West, B. W., & Scott, J. R. (1977) · Virology · doi:10.1016/0042-6822(77)90098-8 · Cited by 26 · see citing works
Site-specific recombinational circularization of bacteriophage P1 DNA
Hochman, L., Segev, N., Sternberg, N. L., & Cohen, G. (1983) · Virology · doi:10.1016/0042-6822(83)90528-7 · Cited by 25 · see citing works
Self-citationA simple technique for the isolation of deletion mutants of phage lambda
Sternberg, N. L., Hamilton, D. L., Enquist, L. W., & Weisberg, R. A. (1979) · Gene · doi:10.1016/0378-1119(79)90006-4 · Cited by 21 · see citing works
Self-citationAbremski, K., & Hoess, R. H. (1983) · Gene · doi:10.1016/0378-1119(83)90166-x · Cited by 16 · see citing works
Self-citation
Flagged first, then by citations
Cited by
Sorted by citations
Gu, H., Zou, Y.-R., & Rajewsky, K. (1993) · Cell · doi:10.1016/0092-8674(93)90644-6 · Cited by 989 · see citing works
Antibody-selectable filamentous fd phage vectors: affinity purification of target genes
Parmley, S. F., & Smith, G. P. (1988) · Gene · doi:10.1016/0378-1119(88)90495-7 · Cited by 871 · see citing works
Miyake, S., Makimura, M., Kanegae, Y., Harada, S., Sato, Y., Takamori, K., Tokuda, C., & Saito, I. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.3.1320 · Cited by 788 · see citing works
Inducible gene targeting in mice using the Cre/lox system.
Sauer, B. (1998) · Methods · doi:10.1006/meth.1998.0593 · Cited by 858 · see citing works
Self-citationIndra, A. K., Warot, X. M., Brocard, J., Bornert, J., Xiao, J. H., Chambon, P., & METZGER, D. (1999) · Nucleic Acids Research · doi:10.1093/nar/27.22.4324 · Cited by 758 · see citing works
Epigenetic Gene Regulation in the Bacterial World
Casadesús, J., & Low, D. A. (2006) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.00016-06 · Cited by 635 · see citing works
Kanegae, Y., Lee, G., Sato, Y., Tanaka, M., Nakal, M., Sakaki, T., Sugano, S., & Saito, I. (1995) · Nucleic Acids Research · doi:10.1093/nar/23.19.3816 · Cited by 606 · see citing works
Brusa, R., Zimmermann, F., Koh, D., Feldmeyer, D., Gass, P., Seeburg, P. H., & Sprengel, R. (1995) · Science · doi:10.1126/science.270.5242.1677 · Cited by 594 · see citing works
Metzger, D., Clifford, J., Chiba, H., & Chambon, P. (1995) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.92.15.6991 · Cited by 570 · see citing works
N6-methyl-adenine: an epigenetic signal for DNA–protein interactions
Wion, D., & Casadesús, J. (2006) · Nature Reviews Microbiology · doi:10.1038/nrmicro1350 · Cited by 527 · see citing works
Sauer, B. (1987) · Molecular and Cellular Biology · doi:10.1128/mcb.7.6.2087 · Cited by 482 · see citing works
Self-citationSimilarities and differences among 105 members of the Int family of site-specific recombinases
Nunes-Düby, S. E., Kwon, H. J., Tirumalai, R. S., Ellenberger, T., & Landy, A. (1998) · Nucleic Acids Research · doi:10.1093/nar/26.2.391 · Cited by 448 · see citing works
Elledge, S. J., Mulligan, J. T., Ramer, S. W., Spottswood, M. R., & Davis, R. W. (1991) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.88.5.1731 · Cited by 345 · see citing works
Mouse Cre-LoxP system: general principles to determine tissue-specific roles of target genes
Kim, H., Kim, M., Im, S.-K., & Fang, S. (2018) · Laboratory Animal Research · doi:10.5625/lar.2018.34.4.147 · Cited by 283 · see citing works
Łobocka, M. B., Rose, D. J., Plunkett, G., Rusin, M., Samojedny, A., Lehnherr, H., Yarmolinsky, M. B., & Blattner, F. R. (2004) · Journal of Bacteriology · doi:10.1128/jb.186.21.7032-7068.2004 · Cited by 308 · see citing works
Sternberg, N. L. (1990) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.87.1.103 · Cited by 264 · see citing works
Self-citationMurphy, J., Mahony, J., Ainsworth, S., Nauta, A., & van Sinderen, D. (2013) · Applied and Environmental Microbiology · doi:10.1128/aem.02229-13 · Cited by 254 · see citing works
[2] Bacteriophage-mediated generalized transduction in Escherichia coli and Salmonella typhimurium
Sternberg, N. L., & Maurer, R. (1991) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/0076-6879(91)04004-8 · Cited by 236 · see citing works
Self-citationAmplification of the <i>neu/erbB-2</i> oncogene in a mouse model of mammary tumorigenesis
Andrechek, E. R., Hardy, W. R., Siegel, P. M., Rudnicki, M. A., Cardiff, R. D., & Muller, W. J. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.97.7.3444 · Cited by 230 · see citing works
Pargellis, C. A., Nunes-Düby, S. E., de Vargas, L. M., & Landy, A. (1988) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)68552-7 · Cited by 190 · see citing works
Cre/ loxP Recombination System and Gene Targeting
Kühn, R., & Torres, R. M. (2003) · Humana Press eBooks · doi:10.1385/1-59259-178-7:175 · Cited by 184 · see citing works
Anton, M., & Graham, F. L. (1995) · Journal of Virology · doi:10.1128/jvi.69.8.4600-4606.1995 · Cited by 184 · see citing works
Siegal, M. L., & Hartl, D. L. (1996) · Genetics · doi:10.1093/genetics/144.2.715 · Cited by 169 · see citing works
Forlino, A., Porter, F. D., Lee, E. J., Westphal, H., & Marini, J. C. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.53.37923 · Cited by 159 · see citing works
Pierce, J. C., Sauer, B., & Sternberg, N. L. (1992) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.89.6.2056 · Cited by 158 · see citing works
Self-citationSauer, B. (2004) · Nucleic Acids Research · doi:10.1093/nar/gkh941 · Cited by 155 · see citing works
Self-citationBigger, B., Tolmachov, O. E., Collombet, J.-M., Fragkos, M., Palaszewski, I., & Coutelle, C. C. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m010873200 · Cited by 149 · see citing works
Poyart, C., Trieu‐Cuot, P., Carlier, C., & Courvalin, P. M. (1989) · The EMBO Journal · doi:10.1002/j.1460-2075.1989.tb08373.x · Cited by 144 · see citing works
Engineered tRNAs suppress nonsense mutations in cells and in vivo
Albers, S., Allen, E. C., Bharti, N., Davyt, M., Joshi, D., Pérez‐García, C. G., Santos, L. A., Mukthavaram, R., Delgado-Toscano, M. A., Molina, B., et al. (2023) · Nature · doi:10.1038/s41586-023-06133-1 · Cited by 140 · see citing works
Iyer, L. M., Zhang, D., Burroughs, A. M., & Aravind, L. (2013) · Nucleic Acids Research · doi:10.1093/nar/gkt573 · Cited by 133 · see citing works
Mori, H., Manabe, T., Watanabe, M., Satoh, Y., Suzuki, N., Toki, S., Nakamura, K., Yagi, T., Kushiya, E., Takahashi, T., et al. (1998) · Neuron · doi:10.1016/s0896-6273(00)80567-x · Cited by 133 · see citing works
Yarmolinsky, M. B., & Sternberg, N. L. (1988) · Springer eBooks · doi:10.1007/978-1-4684-5424-6_9 · Cited by 131 · see citing works
Self-citationMarinus, M., & Løbner‐Olesen, A. (2014) · EcoSal Plus · doi:10.1128/ecosalplus.esp-0003-2013 · Cited by 125 · see citing works
Mou, Z., Wang, X., Fu, Z., Dai, Y., Han, C. D., Ouyang, J., Bao, F., Hu, Y., & Li, J. (2002) · The Plant Cell · doi:10.1105/tpc.001701 · Cited by 125 · see citing works
Methylation of DNA in Prokaryotes
Noyer-Weidner, M., & Trautner, T. A. (1993) · Birkhäuser Basel eBooks · doi:10.1007/978-3-0348-9118-9_4 · Cited by 124 · see citing works
Andreas, S. (2002) · Nucleic Acids Research · doi:10.1093/nar/30.11.2299 · Cited by 123 · see citing works
An array of target-specific screening strains for antibacterial discovery
DeVito, J. A., Mills, J. A., Liu, V. G., Agarwal, A., Sizemore, C. F., Yao, Z., Stoughton, D., Cappiello, M. G., Barbosa, M. D. F. S., Foster, L. A., et al. (2002) · Nature Biotechnology · doi:10.1038/nbt0502-478 · Cited by 114 · see citing works
Gene knock-out technology: a methodological overview for the interested novice
Galli-Taliadoros, L. A., Sedgwick, J. D., Wood, S. A., & Körner, H. (1995) · Journal of Immunological Methods · doi:10.1016/0022-1759(95)00017-5 · Cited by 112 · see citing works
Boccard, F., Smokvina, T., Pernodet, J., Friedmann, A., & Guérineau, M. (1989) · The EMBO Journal · doi:10.1002/j.1460-2075.1989.tb03460.x · Cited by 109 · see citing works
Integration of satellite bacteriophage P4 in Escherichia coli
Pierson, L. S., & Kahn, M. L. (1987) · Journal of Molecular Biology · doi:10.1016/0022-2836(87)90026-x · Cited by 108 · see citing works
Brocard, J. (1998) · Nucleic Acids Research · doi:10.1093/nar/26.17.4086 · Cited by 104 · see citing works
Cre recombinase expression can result in phenotypic aberrations in plants
Coppoolse, E. R., de Vroomen, M. J., Roelofs, D., Smit, J. J. M., van Gennip, F., Hersmus, B. J. M., Nijkamp, H. J. J., & van Haaren, M. J. J. (2003) · Plant Molecular Biology · doi:10.1023/a:1021174726070 · Cited by 93 · see citing works
Ngernprasirtsiri, J., Kobayashi, H., & Akazawa, T. (1988) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.85.13.4750 · Cited by 92 · see citing works
The DIRS1 Group of Retrotransposons
Goodwin, T. J. D., & Poulter, R. T. M. (2001) · Molecular Biology and Evolution · doi:10.1093/oxfordjournals.molbev.a003748 · Cited by 87 · see citing works
Bacterial DNA Methylation and Methylomes
Casadesús, J. (2016) · Advances in experimental medicine and biology · doi:10.1007/978-3-319-43624-1_3 · Cited by 80 · see citing works
Bannam, T. L., Crellin, P. K., & Rood, J. I. (1995) · Molecular Microbiology · doi:10.1111/j.1365-2958.1995.tb02417.x · Cited by 77 · see citing works
Yaar, L., Mevarech, M., & Koltint, Y. (1997) · Microbiology · doi:10.1099/00221287-143-9-3033 · Cited by 76 · see citing works
A mutational analysis of the bacteriophage P1 recombinase Cre
Wierzbicki, A., Kendall, M., Abremski, K., & Hoess, R. H. (1987) · Journal of Molecular Biology · doi:10.1016/0022-2836(87)90484-0 · Cited by 74 · see citing works
Self-citation[47] Using bacteriophage P1 system to clone high molecular weight genomic DNA
Pierce, J. C., & Sternberg, N. L. (1992) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/0076-6879(92)16049-p · Cited by 74 · see citing works
Self-citationCleavage of the bacteriophage P1 packaging site (pac) is regulated by adenine methylation.
Sternberg, N. L., & Coulby, J. N. (1990) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.87.20.8070 · Cited by 72 · see citing works
Self-citationSilencing Inhibits Cre-Mediated Recombination of the Z/AP and Z/EG Reporters in Adult Cells
Long, M. A., & Rossi, F. M. V. (2009) · PLoS ONE · doi:10.1371/journal.pone.0005435 · Cited by 71 · see citing works
The Unconventional Xer Recombination Machinery of Streptococci/Lactococci
Bourgeois, P., Bugarel, M., Campo, N., Daveran‐Mingot, M., Labonté, J., Lanfranchi, D., Lautier, T., Pagès, C., & Ritzenthaler, P. (2007) · PLoS Genetics · doi:10.1371/journal.pgen.0030117 · Cited by 69 · see citing works
Cre Mutants with Altered DNA Binding Properties
Hartung, M., & Kisters–Woike, B. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.36.22884 · Cited by 65 · see citing works
Lymphocyte Ontogeny and Activation in Gene Targeted Mutant Mice
Pfeffer, K., & Mak, T. W. (1994) · Annual Review of Immunology · doi:10.1146/annurev.iy.12.040194.002055 · Cited by 65 · see citing works
DNA specificity of the cre recombinase resides in the 25 kDa carboxyl domain of the protein
Hoess, R. H., Abremski, K., Irwin, S., Kendall, M., & Mack, A. (1990) · Journal of Molecular Biology · doi:10.1016/s0022-2836(99)80007-2 · Cited by 63 · see citing works
Self-citationRecognition and cleavage of the bacteriophage P1 packaging site (pac)
Sternberg, N. L., & Coulby, J. N. (1987) · Journal of Molecular Biology · doi:10.1016/0022-2836(87)90675-9 · Cited by 61 · see citing works
Self-citationFilamentous Fusion Phage Cloning Vectors for the Study of Epitopes and Design of Vaccines
Parmley, S. F., & Smith, G. P. (1989) · Springer eBooks · doi:10.1007/978-1-4757-2046-4_21 · Cited by 61 · see citing works
The cyclization of linear DNA in Escherichia coli by site-specific recombination
Sauer, B., & Henderson, N. (1988) · Gene · doi:10.1016/0378-1119(88)90205-3 · Cited by 61 · see citing works
Self-citationCre-lox recombination in Escherichia coli cells mechanistic differences from the in Vitro reaction
Adams, D. E., Bliska, J. B., & Cozzarelli, N. R. (1992) · Journal of Molecular Biology · doi:10.1016/0022-2836(92)90623-r · Cited by 60 · see citing works
Recognition and cleavage of the bacteriophage P1 packaging site (pac)
Sternberg, N. L., & Coulby, J. N. (1987) · Journal of Molecular Biology · doi:10.1016/0022-2836(87)90674-7 · Cited by 59 · see citing works
Self-citationA Set ofloxPMarker Cassettes for Cre-mediated Multiple Gene Disruption inSchizosaccharomyces pombe
Iwaki, T., & Takegawa, K. (2004) · Bioscience Biotechnology and Biochemistry · doi:10.1271/bbb.68.545 · Cited by 56 · see citing works
Synthesis of FinP RNA by Plasmids F and pSLT Is Regulated by DNA Adenine Methylation
Torreblanca, J., Marqués, S., & Casadesús, J. (1999) · Genetics · doi:10.1093/genetics/152.1.31 · Cited by 56 · see citing works
Oscarsson, J., Karched, M., Thay, B., Chen, C., & Asikainen, S. E. (2008) · BMC Microbiology · doi:10.1186/1471-2180-8-206 · Cited by 55 · see citing works
The Cre-lox Recombination System
Hoess, R. H., & Abremski, K. (1990) · Nucleic acids and molecular biology · doi:10.1007/978-3-642-84150-7_6 · Cited by 55 · see citing works
Self-citationCre/lox: One More Step in the Taming of the Genome
Sauer, B. (2002) · Endocrine · doi:10.1385/endo:19:3:221 · Cited by 55 · see citing works
Self-citationTGF-β superfamily signaling is essential for tooth and hair morphogenesis and differentiation
Klopcic, B. R. S., Maass, T., Meyer, E., Lehr, H. A., METZGER, D., Chambon, P., Mann, A., & Blessing, M. (2007) · European Journal of Cell Biology · doi:10.1016/j.ejcb.2007.03.005 · Cited by 51 · see citing works
The Legacy of Nat Sternberg: The Genesis of Cre-lox Technology
Yarmolinsky, M. B., & Hoess, R. H. (2015) · Annual Review of Virology · doi:10.1146/annurev-virology-100114-054930 · Cited by 50 · see citing works
Self-citationGenetic Analysis of the Mobilization and Leading Regions of the IncN plasmids pKM101 and pCU1
Paterson, E. S., Moré, M. I., Pillay, G., Cellini, C., Woodgate, R., Walker, G. C., Iyer, V. N., & Winans, S. C. (1999) · Journal of Bacteriology · doi:10.1128/jb.181.8.2572-2583.1999 · Cited by 47 · see citing works
Tan, B. T., Wu, L., & Berk, A. J. (1999) · Journal of Virology · doi:10.1128/jvi.73.9.7582-7589.1999 · Cited by 46 · see citing works
Kubo, A., Kusukawa, A., & Komano, T. (1988) · Molecular and General Genetics MGG · doi:10.1007/bf00333394 · Cited by 46 · see citing works
Enhanced cell-permeant Cre protein for site-specific recombination in cultured cells
Lin, Q., Jo, D., Gebre-Amlak, K. D., & Ruley, H. E. (2004) · BMC Biotechnology · doi:10.1186/1472-6750-4-25 · Cited by 44 · see citing works
Roy, S. D., Saxena, M., Bhomkar, P., Pooggin, M. M., Höhn, T., & Sarin, N. B. (2008) · Plant Cell Tissue and Organ Culture (PCTOC) · doi:10.1007/s11240-008-9402-0 · Cited by 41 · see citing works
Sequence-Independent Assembly of Spermatid mRNAs into Messenger Ribonucleoprotein Particles
Schmidt, E. E., Hanson, E. S., & Capecchi, M. R. (1999) · Molecular and Cellular Biology · doi:10.1128/mcb.19.5.3904 · Cited by 41 · see citing works
Role of DNA Methyltransferases in Epigenetic Regulation in Bacteria
Kumar, R., & Rao, D. N. (2012) · Sub-cellular biochemistry/Subcellular biochemistry · doi:10.1007/978-94-007-4525-4_4 · Cited by 39 · see citing works
Choulika, A., Guyot, V., & Nicolas, J. F. (1996) · Journal of Virology · doi:10.1128/jvi.70.3.1792-1798.1996 · Cited by 39 · see citing works
Characterization of the binding sites of c1 repressor of bacteriophage P1
Eliason, J. L., & Sternberg, N. L. (1987) · Journal of Molecular Biology · doi:10.1016/0022-2836(87)90313-5 · Cited by 39 · see citing works
Self-citationMészáros, K., Éva, C., Kiss, T., Bányai, J., Kiss, E., Téglás, F., Láng, L., Karsaï, I., & Tamás, L. (2014) · Plant Molecular Biology Reporter · doi:10.1007/s11105-014-0830-1 · Cited by 38 · see citing works
Sullivan, K. M., & Saunders, J. R. (1988) · Nucleic Acids Research · doi:10.1093/nar/16.10.4369 · Cited by 38 · see citing works
Stimulation of IS1 excision by bacteriophage P1 ref function
Lu, S.-D., Lu, D., & Gottesman, M. E. (1989) · Journal of Bacteriology · doi:10.1128/jb.171.6.3427-3432.1989 · Cited by 36 · see citing works
Sauer, B. (1987) · Molecular and Cellular Biology · doi:10.1128/mcb.7.6.2087-2096.1987 · Cited by 36 · see citing works
Self-citationYu, A., Bertani, L. E., & Haggård‐Ljungquist, E. (1989) · Gene · doi:10.1016/0378-1119(89)90244-8 · Cited by 36 · see citing works
Systematic and scalable genome-wide essentiality mapping to identify nonessential genes in phages
Piya, D., Nolan, N., Moore, M. L., Ramirez Hernandez, L. A., Cress, B. F., Young, R., Arkin, A. P., & Mutalik, V. K. (2023) · PLOS Biology · doi:10.1371/journal.pbio.3002416 · Cited by 31 · see citing works
Modification of the Genome of Rhodobacter sphaeroides and Construction of Synthetic Operons
Jaschke, P. R., Saer, R. G., Noll, S., & Beatty, J. T. (2011) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/b978-0-12-385075-1.00023-8 · Cited by 34 · see citing works
Development of a P1 phagemid system for the delivery of DNA into Gram-negative bacteria
Westwater, C., Schofield, D. A., Schmidt, M. G., Norris, J. S., & Dolan, J. W. (2002) · Microbiology · doi:10.1099/00221287-148-4-943 · Cited by 34 · see citing works
Cao, B., Huang, Z., Chen, G., & Lei, J. (2010) · Genetics and Molecular Biology · doi:10.1590/s1415-47572010005000043 · Cited by 34 · see citing works
Bestor, A. C., Stewart, P. E., Jewett, M. W., Sarkar, A., Tilly, K., & Rosa, P. A. (2010) · Infection and Immunity · doi:10.1128/iai.01059-09 · Cited by 31 · see citing works
Iida, S., Hiestand-Nauer, R., Sandmeier, H., Lehnherr, H., & Arber, W. (1998) · Virology · doi:10.1006/viro.1998.9405 · Cited by 30 · see citing works
Random segment deletion based on IS31831 and Cre/loxP excision system in Corynebacterium glutamicum
Tsuge, Y., Suzuki, N., Inui, M., & Yukawa, H. (2007) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-006-0788-5 · Cited by 29 · see citing works
Fujise, O., Wang, Y., Chen, W., & Chen, C. (2008) · Oral Microbiology and Immunology · doi:10.1111/j.1399-302x.2007.00416.x · Cited by 28 · see citing works
Use of Site-Specific Recombination Systems in Plants
Odell, J. T., & Russell, S. H. (1994) · Springer eBooks · doi:10.1007/978-94-011-1094-5_11 · Cited by 28 · see citing works
Krappmann, S., Pries, R., Gellissen, G., Hiller, M., & Braus, G. H. (2000) · Journal of Bacteriology · doi:10.1128/jb.182.15.4188-4197.2000 · Cited by 28 · see citing works
Engineering Embryonic Stem Cells with Recombinase Systems
Schnütgen, F., Stewart, A. F., von Melchner, H., & Anastassiadis, K. (2006) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/s0076-6879(06)20007-7 · Cited by 27 · see citing works
Production of a reporter transgenic pig for monitoring Cre recombinase activity
Li, L., Pang, D., Wang, T., Li, Z., Chen, L., Zhang, M., Song, N., Nie, D., Chen, Z., Lai, L., et al. (2009) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2009.02.146 · Cited by 27 · see citing works
The c1 repressor of bacteriophage P1. Isolation and characterization of the repressor protein.
Dreiseikelmann, B., Velleman, M., & Schuster, H. (1988) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)57316-1 · Cited by 26 · see citing works
Ahlstrand, T., Tuominen, H. K., Beklen, A., Torittu, A., Oscarsson, J., Sormunen, R. T., Pöllänen, M. T., Permi, P., & Ihalin, R. (2016) · Virulence · doi:10.1080/21505594.2016.1216294 · Cited by 25 · see citing works
Genetic analysis of the lytic replicon of bacteriophage P1
Sternberg, N. L., & Cohen, G. (1989) · Journal of Molecular Biology · doi:10.1016/0022-2836(89)90444-0 · Cited by 25 · see citing works
Self-citationInducible gene expression systems inLactococcus lactis
Djordjevic, G. M., & Klaenhammer, T. R. (1998) · Molecular Biotechnology · doi:10.1007/bf02760814 · Cited by 24 · see citing works
Vector Systems for Prenatal Gene Therapy: Principles of Non-viral Vector Design and Production
Wong, S. P., Argyros, O., & Harbottle, R. P. (2012) · Methods in molecular biology · doi:10.1007/978-1-61779-873-3_7 · Cited by 23 · see citing works
Organization of the immunity region immI of bacteriophage P1 and synthesis of the P1 antirepressor
Heisig, A., Riedel, H.-D., Dobrinski, B., Lurz, R., & Schuster, H. (1989) · Journal of Molecular Biology · doi:10.1016/0022-2836(89)90591-3 · Cited by 23 · see citing works
Sato, M., Watanabe, T., Oshida, A., Nagashima, A., Miyazaki, J., & Kimura, M. (2001) · Molecular Reproduction and Development · doi:10.1002/mrd.1109 · Cited by 22 · see citing works
- Authors
- Sternberg, N., Sauer, B., Hoess, R., & Abremski, K.
- Affiliations
- Frederick National Laboratory for Cancer Research (US)
- Venue
- Journal of Molecular Biology
- Publisher
- Elsevier BV
- Published
- 1986-01
- Volume
- 187
- Issue
- 2
- Pages
- 197-212
- ISSN
- 0022-2836 (ISSN-L)
- OpenAlex
- W1594256225
- Cited by
- 171
- References
- 36
- Citations by year
- 2026: 2 · 2025: 2 · 2024: 2 · 2023: 5 · 2022: 3 · 2020: 3 · 2019: 1 · 2018: 2 · 2017: 1 · 2016: 4 · 2015: 2 · 2014: 3 · 2013: 7 · 2012: 4
- FWCI
- 20.35
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Molecular Biology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (n01co023909-011)
- National Cancer Institute (N01-CO23909)
- Topics
- Physical Sciences › Environmental Science › Ecology › Bacteriophages and microbial interactions
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Keywords
- bacteriophage P1, Cre recombinase, promoter, Dam methylation, RecA, lysogeny, vegetative growth, viral life cycle
- MeSH terms
- Base Sequence, Coliphages, DNA Nucleotidyltransferases, DNA, Viral, Gene Expression Regulation, Genes, Genes, Regulator, Genes, Viral, Lysogeny, Methylation, Mutation, Plasmids, Promoter Regions, Genetic, Suppression, Genetic, Transcription, Genetic, Protein Biosynthesis, Viral Proteins, Integrases
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