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Cadmium transport across tonoplast of vesicles from oat roots. Evidence for a Cd2+/H+ antiport activity
Open accessAbstract
Cadmium accumulates in the vacuole of plant cells, but the mechanism driving its transport across the vacuole membrane is not understood. Here we present evidence for Cd2+ transport via a Cd2+/H+ antiport activity into tonoplast-enriched vesicles isolated from oat roots. Experimentally, accumulation of Cd2+ into vesicles could be driven by delta pH generated by either V-type ATPase or artificially using nigericin to exchange K+ and H+ in K(+)-loaded vesicles. When tonoplast-enriched vesicles were separated on a linear sucrose gradient, NO3(-)-sensitive ATPase, total MgATPase, and delta pH-dependent Cd2+ transport equilibrated at 1.11 g/ml, the density of tonoplast membrane. Cd2+ accumulation in vesicles was accompanied by efflux of protons in a Cd2+ concentration-dependent manner characteristic of an antiport activity. The delta pH-dependent Cd2+ accumulation process showed saturation kinetics with a Km(app) of 5.5 microM. Thus the process is a candidate for transport of Cd2+ from the cytoplasm to the vacuolar sap under conditions of low as well as high Cd2+ exposure.
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Citations
Cited by
Molecular mechanisms of plant metal tolerance and homeostasis
Clemens, S. (2001) · Planta · doi:10.1007/s004250000458 · Cited by 1,417
The ZIP family of metal transporters
Guerinot, M. L. (2000) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/s0005-2736(00)00138-3 · Cited by 1,200
Tolerance to toxic metals by a gene family of phytochelatin synthases from plants and yeast
Clemens, S., Kim, E. J., Neumann, D., & Schroeder, J. I. (1999) · The EMBO Journal · doi:10.1093/emboj/18.12.3325 · Cited by 660
Engineering tolerance and accumulation of lead and cadmium in transgenic plants
Song, W.-Y., Ju Sohn, E., Martinoia, E., Jik Lee, Y., Yang, Y.-Y., Jasinski, M., Forestier, C., Hwang, I., & Lee, Y. (2003) · Nature Biotechnology · doi:10.1038/nbt850 · Cited by 423
Grant, C. A., Buckley, W. T., Bailey, L. D., & Selles, F. (1998) · Canadian Journal of Plant Science · doi:10.4141/p96-100 · Cited by 382
Metadata
- Authors
- Salt, D. E., & Wagner, G. J.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1993-06
- Volume
- 268
- Issue
- 17
- Pages
- 12297-12302
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1545108295
- Cited by
- 212
- References
- 0
- FWCI
- 1.87
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- CoChat. (2026). Citation check: Cadmium transport across tonoplast of vesicles from oat roots. Evidence for a Cd2+/H+ antiport activity. https://app.cochat.ai/papers/10.1016/s0021-9258(18)31388-7
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- Salt, D. E., & Wagner, G. J. (1993). Cadmium transport across tonoplast of vesicles from oat roots. Evidence for a Cd2+/H+ antiport activity. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(18)31388-7
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