Cited by 506Glycosylation and Glycoproteins ResearchCarbohydrate Chemistry and SynthesisGalectins and Cancer Biology

Binding of synthetic oligosaccharides to the hepatic Gal/GalNAc lectin. Dependence on fine structural features.

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Lee, Y C, Townsend, R R, Hardy, M R, Lönngren, J, Arnarp, J, Haraldsson, M, Lönn, H·1983·Journal of Biological Chemistry

Abstract

A series of synthetic oligosaccharides, resembling natural N-acetyllactosamine type glycans, were tested for their ability to inhibit the binding of labeled ligand to the mammalian hepatic lectin on rabbit hepatocytes at 2 degrees C. A dramatic hierarchy of inhibitory potency (tetraantennary greater than triantennary much greater than biantennary much greater than monoantennary) could be demonstrated. The range of concentration required for 50% inhibition of labeled ligand binding extended from approximately 1 mM, for the monoantennary oligosaccharides, to approximately 1 nM for triantennary oligosaccharides, even though the absolute Gal concentration increased only 3-fold. It was found that the number of Gal residues/cluster and their branching mode are major determinants of binding affinity of ligands to the hepatic lectin on the surface of hepatocytes.

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Crossref · 10.1016/s0021-9258(18)33240-x

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24 of 24 references verified0 citation errors · 0 not checkable

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Authors
Lee, Y. C., Townsend, R. R., Hardy, M. R., Lönngren, J., Arnarp, J., Haraldsson, M., & Lönn, H.
Affiliations
Johns Hopkins University (US)
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
1983-01
Volume
258
Issue
1
Pages
199-202
ISSN
0021-9258 (ISSN-L)
OpenAlex
W1595570412
Cited by
503
References
24
Citations by year
2026: 7 · 2025: 6 · 2024: 10 · 2023: 18 · 2022: 11 · 2021: 15 · 2020: 17 · 2019: 9 · 2018: 10 · 2017: 4 · 2016: 7 · 2015: 13 · 2014: 14 · 2013: 13 · 2012: 19
FWCI
5.24
Type
journal article
Language
en
Open access
Locations
  • Journal of Biological Chemistry (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Glycosylation and Glycoproteins Research
  • Physical Sciences › Chemistry › Organic Chemistry › Carbohydrate Chemistry and Synthesis
  • Life Sciences › Immunology and Microbiology › Immunology › Galectins and Cancer Biology
Keywords
Gal/GalNAc lectin, synthetic oligosaccharides, N-acetyllactosamine, oligosaccharide branching, hepatocytes
MeSH terms
Animals, Binding, Competitive, Carbohydrate Sequence, Cell Membrane, Glycopeptides, Kinetics, Liver, Oligosaccharides, Rabbits, Structure-Activity Relationship, Lectins

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