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Guanylate cyclase from bovine lung. A kinetic analysis of the regulation of the purified soluble enzyme by protoporphyrin IX, heme, and nitrosyl-heme.
Open accessAbstract
A kinetic characterization of the regulation of purified soluble guanylate cyclase from bovine lung by protoporphyrin M and hematin is reported.Purified guanylate cyclase was isolated with heme and had specific activities @mol of cGMP/min/mg of protein) of 0.1-0.2 and 0.3-0.6 in the presence of excess MgGTP and MnGTP, respectively, in the absence of added activators.Protoporphyrin IX, nitric oxide (NO), and NOheme increased the V,, up to 40-fold and decreased the K,,, for GTP (from 100 to 45-55 p ~) in the presence of excess Mg'.However, in the presence of excess Mn2+ the V,, was increased only slightly and the K , for GTP was unchanged.Protoporphyrin IX resembled NO and NO-heme also in lowering the K , and Ki for uncomplexed metal.This close similarity in the interactions of these activators with guanylate cyclase suggests that a common form of activated enzyme is generated.Hematin, in excess of 1.5 p ~, inhibited guanylate cyclase activity.Smaller concentrations of hematin competitively inhibited protoporphyrin IX (KI = 0.35 p ~) ,
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Ignarro, L. J., Lippton, H., Edwards, J. C., Baricos, W. H., Hyman, A. L., Kadowitz, P. J., & Gruetter, C. A. (1981) · The Journal of Pharmacology and Experimental Therapeutics · doi:10.1016/s0022-3565(25)32738-2 · Cited by 1,633
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Cited by
Biosynthesis and Metabolism of Endothelium-Derived Nitric Oxide
Ignarro, L. J. (1990) · Annual Review of Pharmacology and Toxicology · doi:10.1146/annurev.pa.30.040190.002535 · Cited by 1,314
Self-citationGuanylate cyclase and the ⋅NO/cGMP signaling pathway
Denninger, J. W., & Marletta, M. A. (1999) · Biochimica et Biophysica Acta (BBA) - Bioenergetics · doi:10.1016/s0005-2728(99)00024-9 · Cited by 1,040
Stone, J. R., & Marletta, M. A. (1994) · Biochemistry · doi:10.1021/bi00184a036 · Cited by 647
Nitric Oxide: A Unique Endogenous Signaling Molecule in Vascular Biology
Ignarro, L. J. (1999) · Bioscience Reports · doi:10.1023/a:1020150124721 · Cited by 304
Self-citationConnecting the Chemical and Biological Properties of Nitric Oxide
Toledo, J. C., & Augusto, O. (2012) · Chemical Research in Toxicology · doi:10.1021/tx300042g · Cited by 286
Metadata
- Authors
- Wolin, M. S., Wood, K. S., & Ignarro, L. J.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1982-11
- Volume
- 257
- Issue
- 22
- Pages
- 13312-13320
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W105256667
- Cited by
- 185
- References
- 1
- FWCI
- 2.28
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- CoChat. (2026). Citation check: Guanylate cyclase from bovine lung. A kinetic analysis of the regulation of the purified soluble enzyme by protoporphyrin IX, heme, and nitrosyl-heme. https://app.cochat.ai/papers/10.1016/s0021-9258(18)33448-3
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- Wolin, M. S., Wood, K. S., & Ignarro, L. J. (1982). Guanylate cyclase from bovine lung. A kinetic analysis of the regulation of the purified soluble enzyme by protoporphyrin IX, heme, and nitrosyl-heme. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(18)33448-3
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