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Precise location of two promoters for the beta-lactamase gene of pBR322. S1 mapping of ribonucleic acid isolated from Escherichia coli or synthesized in vitro.
Open accessAbstract
We identified two promoters for the beta-lactamase gene of plasmid pBR322. RNA isolated from bacteria containing pBR322 or RNA transcribed in vitro on pBR322 templates was hybridized to 5' end-labeled single-stranded plasmid probes (Berk, A. J., and Sharp, P. A. (1977) Cell 12, 721-732). Electrophoretic analysis of the nuclease S1 digestion products next to Maxam-Gilbert sequencing ladders closely defines the transcriptional initiation points. The natural promoter lies near the coding sequence of the beta-lactamase gene, initiating transcription at -35 bases before the ATG initiation codon, while a second promoter initiates at positions -244 and/or -245 (on the opposite side of the Eco RI site). This promoter overlaps the promoter transcribing in the opposite direction toward the tetracycline gene(s) and starts in the -10 region of that promoter. S1 mapping of procaryotic mRNA, transcribed in vivo, allows both an accurate identification of promoters and the analysis of their transcriptional regulation.
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Citations
Cited by
TnphoA: a transposon probe for protein export signals.
Manoil, C., & Beckwith, J. (1985) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.82.23.8129 · Cited by 1,013
Transcriptional Regulator of Oxidative Stress-Inducible Genes: Direct Activation by Oxidation
Storz, G., Tartaglia, L. A., & Ames, B. N. (1990) · Science · doi:10.1126/science.2183352 · Cited by 874
Lawrence, C. E., & Reilly, A. A. (1990) · Proteins: Structure, Function, and Bioinformatics · doi:10.1002/prot.340070105 · Cited by 526
MECHANISMS UNDERLYING EXPRESSION OF TN 10 ENCODED TETRACYCLINE RESISTANCE
Hillen, W., & Berens, C. (1994) · Annual Review of Microbiology · doi:10.1146/annurev.mi.48.100194.002021 · Cited by 511
Compilation and analysis of DNA sequences associated with apparent streptomycete promoters
Strohl, W. R. (1992) · Nucleic Acids Research · doi:10.1093/nar/20.5.961 · Cited by 477
Metadata
- Authors
- Brosius, J., Cate, R. L., & Perlmutter, A. P.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1982-08
- Volume
- 257
- Issue
- 15
- Pages
- 9205-9210
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1645072707
- Cited by
- 211
- References
- 0
- FWCI
- 17.59
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- CoChat. (2026). Citation check: Precise location of two promoters for the beta-lactamase gene of pBR322. S1 mapping of ribonucleic acid isolated from Escherichia coli or synthesized in vitro. https://app.cochat.ai/papers/10.1016/s0021-9258(18)34261-3
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- Brosius, J., Cate, R. L., & Perlmutter, A. P. (1982). Precise location of two promoters for the beta-lactamase gene of pBR322. S1 mapping of ribonucleic acid isolated from Escherichia coli or synthesized in vitro. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(18)34261-3
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
