The DOI matches its Crossref record; 60 of the 60 references it cites were found in a registry or an index.
Characterization of the promoter for vascular cell adhesion molecule-1 (VCAM-1).
Open accessAbstract
Vascular cell adhesion molecule-1 (VCAM-1) was first identified as a protein that appears on the surface of endothelial cells after exposure to inflammatory cytokines. Through interaction with its integrin counter receptor VLA-4, VCAM-1 mediates cell-cell interactions important for immune function. We have cloned and begun characterization of the promoter for the VCAM-1 gene. In a series of transfection assays into human umbilical vein endothelial cells (HUVECs), we find that silencers between positions -1.641 kilobases and -288 base pairs restrict promoter activity, and that treatment with tumor necrosis factor-alpha overcomes this inhibition and activates the promoter through two NF kappa B sites located at positions -77 and -63 base pairs of the VCAM-1 gene. This responsiveness appears cell-specific since constructs containing the VCAM-1 NF kappa B sites are not responsive to tumor necrosis factor alpha in the T-cell line Jurkat. The two VCAM-1 NF kappa B sites, which differ slightly in their sequence, form distinct complexes in gel retardation assays, suggesting that they interact with different NF kappa B-site binding proteins. The distribution of these proteins could then control activity of the NF kappa B sites. We conclude that the pattern of VCAM-1 expression in HUVECs is controlled by a combination of these silencers and NF kappa B sites.
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Metadata
- Authors
- Iademarco, M. F., McQuillan, J. J., Rosen, G. D., & Dean, D. C.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1992-08
- Volume
- 267
- Issue
- 23
- Pages
- 16323-16329
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1506569093
- Cited by
- 473
- References
- 60
- FWCI
- 5.45
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Iademarco, M. F., McQuillan, J. J., Rosen, G. D., & Dean, D. C. (1992). Characterization of the promoter for vascular cell adhesion molecule-1 (VCAM-1). Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(18)42004-2
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
