The DOI matches its Crossref record; 48 of the 48 references it cites were found in a registry or an index.
Induction of Sp1-p53 DNA-binding heterocomplexes during granulocyte/macrophage colony-stimulating factor-dependent proliferation in human erythroleukemia cell line TF-1.
Open accessAbstract
The involvement of Sp1 in regulating cell proliferation in myeloid leukemia cells was determined by measuring the levels and DNA binding activity of Sp1 in TF-1 cells, a human erythroleukemia cell line dependent on granulocyte/macrophage colony-stimulating factor (GM-CSF) for viability and cell growth. DNA binding of Sp1 to a specific double-stranded oligodeoxynucleotide was increased markedly in a dose-dependent manner in proliferating cells in response to GM-CSF compared with growth-arrested or apoptotic cells. Competition experiments and mobility shift interference assays with antibodies against Sp1 as well as wild-type or mutant p53 indicated that GM-CSF-inducible DNA-binding complexes contained both Sp1 and p53 and that these heterocomplexes bound to both p53- and Sp1-binding sequences with high affinity. Immunoprecipitation of nuclear extracts with a p53 antibody indicated that Sp1 was associated as a heterocomplex with p53. Formation of this complex was dependent on the level of p53 since p53 was more abundant in proliferating cells and decreased upon induction of growth arrest and apoptosis by withdrawal of GM-CSF while Sp1 levels remained unchanged. These results suggest that the association of Sp1 with p53 may represent a novel mechanism of growth regulation in cytokine-dependent leukemia cells.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“In 1993, p53 was first reported to bind to coactivators, including the TATA-box binding protein,73, 74 the CCAAT-box binding factor (NF-Y)75 and specificity protein 1 (Sp1) that binds to GC-boxes,76 and to interfere with their transactivator function.”
From Census and evaluation of p53 target genes (2017) · cites this paper as [76]
Citations
Cites
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
LAEMMLI, U. K. (1970) · Nature · doi:10.1038/227680a0 · Cited by 249,961
Glucocorticoid-induced thymocyte apoptosis is associated with endogenous endonuclease activation
Wyllie, A. H. (1980) · Nature · doi:10.1038/284555a0 · Cited by 4,499
The p53 tumour suppressor gene
Levine, A. J., Momand, J. A., & Finlay, C. A. (1991) · Nature · doi:10.1038/351453a0 · Cited by 3,785
Transcriptional Regulation in Mammalian Cells by Sequence-Specific DNA Binding Proteins
Mitchell, P. J., & Tjian, R. (1989) · Science · doi:10.1126/science.2667136 · Cited by 3,046
T antigen is bound to a host protein in SY40-transformed cells
Lane, D. P., & Crawford, L. V. (1979) · Nature · doi:10.1038/278261a0 · Cited by 2,456
Andrews, N. C., & Faller, D. V. (1991) · Nucleic Acids Research · doi:10.1093/nar/19.9.2499 · Cited by 2,292
Fos and jun: The AP-1 connection
Curran, T., & Franza, B. R. (1988) · Cell · doi:10.1016/0092-8674(88)90024-4 · Cited by 1,595
Regulation of Inducible and Tissue-Specific Gene Expression
Maniatis, T., Goodbourn, S., & Fischer, J. A. (1987) · Science · doi:10.1126/science.3296191 · Cited by 1,553
Dynan, W. S., & Tjian, R. (1983) · Cell · doi:10.1016/0092-8674(83)90210-6 · Cited by 1,445
Imagawa, M. (1987) · Cell · doi:10.1016/0092-8674(87)90152-8 · Cited by 1,384
Research works printed but not linked: 7
- Borellini, 1990, Mol. Cell. Biol., 10, 5541
- Kim, 1992, Mol. Cell. Biol., 12, 2455
- Koury, 1992, Exp. Hematol., 20, 391
- Mercer, 1984, Mol. Cell. Biol., 4, 276
- Nakamura, 1991, Cell Growth & Differ., 2, 267
- Shabo, 1990, Leukemia, 4, 797
- Zhang, 1992, Oncogene, 7, 1039
Cited by
Sorted by citations
Items 101–147 of 147
p53-dependent downregulation of metastasis-associated laminin receptor
Modugno, M., Tagliabue, E., Ardini, E., Berno, V., Galmozzi, E., Bortoli, M. D., Castronovo, V., & Ménard, S. (2002) · Oncogene · doi:10.1038/sj.onc.1205957 · Cited by 28
Transcriptional Regulation by p53
Hsu, Y.-S., Tang, F.-M., Liu, W., Chuang, J., Lai, M., & Lin, Y.-S. (1995) · Journal of Biological Chemistry · doi:10.1074/jbc.270.12.6966 · Cited by 28
Mutant p53 and ETS2, a Tale of Reciprocity
Martinez, L. A. (2016) · Frontiers in Oncology · doi:10.3389/fonc.2016.00035 · Cited by 26
Repression of Human Reduced Folate Carrier Gene Expression by Wild Type p53
Ding, B. C., Whetstine, J. R., Witt, T. L., Schuetz, J. D., & Matherly, L. H. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m005248200 · Cited by 26
Chang, N.-T., Yang, W., Huang, H.-C., Yeh, K., & Wu, C.-W. (2006) · Journal of Biomedical Science · doi:10.1007/s11373-006-9126-2 · Cited by 25
Keating, K. E., Gueven, N., Watters, D. J., Rodemann, H. P., & Lavin, M. F. (2001) · Oncogene · doi:10.1038/sj.onc.1204527 · Cited by 25
Transcriptional regulation of multidrug resistance in breast cancer
Glazer, R. I., & Rohlff, C. (1994) · Breast Cancer Research and Treatment · doi:10.1007/bf00666159 · Cited by 25
Self-citationRoemer, K., & Friedmann, T. (1994) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.1994.tb21718.x · Cited by 24
Gately, D. P., & Howell, S. B. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.34.20588 · Cited by 22
Zhang, D., Cao, D., Russell, R. L., & Pizzorno, G. (2001) · PubMed · Cited by 20
A novel network-based method for measuring the functional relationship between gene sets
Wang, Q., Sun, J., Zhou, M., Yang, H., Li, Y., Li, X., Lv, S., Li, Y., & Li, Y. (2011) · Bioinformatics · doi:10.1093/bioinformatics/btr154 · Cited by 19
p53 regulates Ki-67 promoter activity through p53- and Sp1-dependent manner in HeLa cells
Wang, M., Pei, D., Qian, G., Yin, X., Cheng, Q., Li, L., Li, H., & Zheng, J. (2011) · Tumor Biology · doi:10.1007/s13277-011-0191-4 · Cited by 18
Joshi, A. A., Wu, Z., Reed, R. F., & Suttle, D. P. (2003) · Molecular Pharmacology · doi:10.1124/mol.63.2.359 · Cited by 18
Too, C. K. L. (1997) · Molecular and Cellular Endocrinology · doi:10.1016/s0303-7207(97)04019-7 · Cited by 18
Vaughan, C. A., Deb, S. P., Deb, S., & Windle, B. E. (2014) · Oncotarget · doi:10.18632/oncotarget.1708 · Cited by 17
Urashima, M., Teoh, G. K. H., Chauhan, D., Ogata, A., Shirahama, S., Kaihara, C., Matsuzaki, M., Matsushima, H., Akiyama, M., Yuza, Y., et al. (1998) · Blood · doi:10.1182/blood.v92.3.959 · Cited by 14
Yu, Z. (2004) · World Journal of Gastroenterology · doi:10.3748/wjg.v10.i13.1979 · Cited by 14
Uchida, C., Oda, T., Sugiyama, T., Otani, S., Kitagawa, M., & Ichiyama, A. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m201380200 · Cited by 13
Watanabe, S., Ito, Y., Miyajima, A., & Arai, K. (1995) · Journal of Biological Chemistry · doi:10.1074/jbc.270.16.9615 · Cited by 13
In vitro toxicity of 7H-dibenzo[c,g]carbazole in human liver cell lines
O’Brien, T. J., Schneider, J., Warshawsky, D., & Mitchell, K. R. (2002) · Toxicology in Vitro · doi:10.1016/s0887-2333(02)00013-9 · Cited by 12
Torgeman, A., Mor‐Vaknin, N., & Aboud, M. (1999) · Virology · doi:10.1006/viro.1998.9556 · Cited by 12
Molecular cloning and preliminary analysis of the human α-methylacyl-CoA racemase promoter
Chen, W., Wu, W., Zhao, J., Yu, C., Liu, W., Jiang, A., & Zhang, J. (2007) · Molecular Biology Reports · doi:10.1007/s11033-007-9196-x · Cited by 10
p53 down‐regulates human bradykinin B1 receptor gene expression
Yang, X., Taylor, L., & POLGAR, P. R. (2001) · Journal of Cellular Biochemistry · doi:10.1002/jcb.1117 · Cited by 11
p53 Represses Transcription of RING Finger LIM Domain-Binding Protein RLIM through Sp1
Kong, X., Peng, B., Yang, Y., Zhang, P., Qin, B., Han, D., Wang, C., Dang, Y., Liu, J. O., & Yu, L. (2013) · PLoS ONE · doi:10.1371/journal.pone.0062832 · Cited by 10
A CCACC Motif Mediates Negative Transcriptional Regulation of the Human Erythropoietin Receptor
Maouche, L., Lucien, N., Cartron, J., & Chrétien, S. (1995) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1995.793_3.x · Cited by 10
Increased expression of human reduced folate carrier in fetal Down syndrome brain
Lübec, G., Bajo, M., Cheon, M. S., Bajova, H., & Matherly, L. H. (2003) · Journal of neural transmission. Supplementum · doi:10.1007/978-3-7091-6721-2_8 · Cited by 9
Pathways of p53-Dependent Apoptosis
Grasso, L., & Mercer, W. E. (1997) · Vitamins and hormones · doi:10.1016/s0083-6729(08)60706-2 · Cited by 8
Jackson, P., Shield, M. A., Buskin, J. N., Hawkes, S. P., Reed, M. J., Perrem, K., Hauschka, S. D., & Braithwaite, A. W. (1995) · Europe PMC (PubMed Central) · Cited by 8
Song, K., Kim, Y.-S., Moon, S., Ko, C., Cho, K., Bae, H.-S., & Lee, K.-S. (2001) · The American Journal of Chinese Medicine · doi:10.1142/s0192415x01000460 · Cited by 7
Posttranslational Mechanisms Leading to Mammalian Gene Activation in Response to Genotoxic Stress
Liu, Y., Gorospe, M., Holbrook, N. J., & Anderson, C. W. (1998) · Humana Press eBooks · doi:10.1007/978-1-59259-455-9_15 · Cited by 7
p53 overexpression represses androgen-mediated induction of NKX3.1 in a prostate cancer cell line
Jiang, A., Yu, C., Zhang, P., Chen, W., Liu, W., Hu, X., & Zhang, J. (2006) · Experimental & Molecular Medicine · doi:10.1038/emm.2006.74 · Cited by 6
Ko, S., Lee, K.-S., Cho, K., Kim, Y.-S., Bae, H.-S., & Moon, S. (2000) · The American Journal of Chinese Medicine · doi:10.1142/s0192415x00000088 · Cited by 5
p53 Modulation of DNA Topoisomerase IIα Gene Expression
Joshi, A. A., Wang, Q., & Suttle, D. P. (2000) · Cell Biochemistry and Biophysics · doi:10.1385/cbb:33:2:209 · Cited by 3
Polymorphisms in promoter sequences of MDM2, p53, and p16INK4a genes in normal Japanese individuals
Ohsaka, Y., & Nishino, H. (2010) · Genetics and Molecular Biology · doi:10.1590/s1415-47572010000400004 · Cited by 3
Basha, R. M., Wei, W., Reddy, G. R., & Zawia, N. (2004) · Informa Healthcare eBooks · doi:10.1201/9780203503317.ch3 · Cited by 2
Patho)physiological function of the Multidrug Resistance protein MRP1
Renes, J. W. (2000) · University of Groningen research database (University of Groningen / Centre for Information Technology) · Cited by 1
Moon, S., Jin, H.-S., Ko, C., Kim, Y., Bae, H.-S., Lee, K.-S., & Cho, K. (2005) · Journal of Ethnopharmacology · doi:10.1016/j.jep.2005.02.032 · Cited by 1
Retinoblastoma Protein, Gene Expression, and Cell Cycle Control
Azizkhan, J. C., Lin, S. Y., Jensen, D. E., Kostic, D., & Black, A. R. (1996) · Springer eBooks · doi:10.1007/978-1-4615-5895-8_10
Gain‐of‐Function Mutants of p53 and Their Role in Tumourigenesis
Vaughan, C. A., Deb, S. P., & Deb, S. (2014) · Encyclopedia of Life Sciences · doi:10.1002/9780470015902.a0022449
Ohsaka, Y., & Nishino, H. (2011) · Genes & Genomics · doi:10.1007/s13258-011-0085-2
in normal Japanese individuals
Ohsaka, Y., & Nishino, H. (2010)
Basha, W. W. M. R. (2004) · CRC Press eBooks · doi:10.1201/9780203503317-7
Regulation of Sp2 DNA-Binding Activity and trans-Activation
Moorefield, K. S. (2005) · NCSU Libraries Repository (North Carolina State University Libraries)
Hesketh, R. (1997) · Elsevier eBooks · doi:10.1016/b978-012344548-3/50085-6
The Role of miRNAs and Alternative Polyadenylation in Modulating the P53 Response
Browning, J. W. L. (2018) · Carleton University's Institutional Repository (MacOdrum Library, Carleton University) · doi:10.22215/etd/2018-13260
Unraveling the role of specificity protein 1 in gliomas: pathophysiology and clinical implications
Naseri, A., Razi, S., & Rezaei, N. (2025) · Clinical & Translational Oncology · doi:10.1007/s12094-025-04058-x
Miao (1999) · doi:10.1002/(sici)1097-0215(19991029)83:3
Metadata
- Authors
- Borellini, F., & Glazer, R. I.
- Affiliations
- Georgetown University Medical Center (US)
- ORCID
- Glazer, R.I.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1993-04
- Volume
- 268
- Issue
- 11
- Pages
- 7923-7928
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1547769073
- Cited by
- 147
- References
- 48
- Citations by year
- 2025: 1 · 2022: 1 · 2018: 1 · 2017: 2 · 2016: 3 · 2014: 4 · 2013: 1 · 2012: 2
- FWCI
- 4.98
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5r01ca054231-03)
- National Cancer Institute (CA54231)
- Topics
- Health Sciences › Medicine › Oncology › Cancer-related Molecular Pathways
- Health Sciences › Medicine › Hematology › Acute Myeloid Leukemia Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Keywords
- Sp1, p53, heterocomplexes, DNA binding, GM-CSF, erythroleukemia, TF-1 cell line, cell proliferation, growth regulation, apoptosis, growth arrest, transcription factor binding sites
- MeSH terms
- Base Sequence, Cell Division, Cell Nucleus, Chromatin, DNA-Binding Proteins, DNA, Neoplasm, Leukemia, Erythroblastic, Acute, Humans, Microscopy, Electron, Molecular Sequence Data, Oligodeoxyribonucleotides, Tumor Cells, Cultured, Tumor Suppressor Protein p53, Granulocyte-Macrophage Colony-Stimulating Factor, Sp1 Transcription Factor, Apoptosis
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review