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Identification of substrate recognition determinants for human ERK1 and ERK2 protein kinases.
Open accessAbstract
Two epidermal growth factor-stimulated protein kinases that correspond to ERK1 and ERK2 have been purified from human epidermoid carcinoma cells (Northwood, I. C., Gonzalez, F. A., Wartmann, M., Raden, D. L., and Davis, R. J. (1991) J. Biol. Chem. 266, 15266-15276). A consensus primary sequence for substrates of ERK1 has been identified as -Pro-Leu-Ser/Thr-Pro- (Alvarez, E., Northwood, I. C., Gonzalez, F. A., Latour, D. A., Seth, A., Abate, C., Curran, T., and Davis, R. J. (1991) J. Biol. Chem. 266, 15277-15285). However, the structural determinants for substrate recognition are not understood. We performed a systematic analysis of the effect of point mutations in the primary sequence of peptide substrates on the rate of phosphorylation by ERK1 and ERK2. The results of this investigation demonstrate that the substrate specificities of the ERK1 and ERK2 protein kinases are very similar. We propose that the primary sequence of substrates for ERK1 and ERK2 protein kinases can be generalized as -Pro-Xaan-Ser/Thr-Pro- (where Xaa is a neutral or basic amino acid and n = 1 or 2).
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Cited by
Dérijard, B., Hibi, M., Wu, I.-H., Barrett, T., Su, B., Deng, T., Karin, M., & Davis, R. J. (1994) · Cell · doi:10.1016/0092-8674(94)90380-8 · Cited by 3,188
Self-citationWen, Z., Zhong, Z., & Darnell, J. E. (1995) · Cell · doi:10.1016/0092-8674(95)90311-9 · Cited by 1,974
Activation of the estrogen receptor through phosphorylation by mitogen-activated protein kinase
Kato, S., Endoh, H., Masuhiro, Y., Kitamoto, T., Uchiyama, S., Sasaki, H., Masushige, S., Gotoh, Y., Nishida, E., Kawashima, H., et al. (1995) · Science · doi:10.1126/science.270.5241.1491 · Cited by 1,964
Inhibition of Adipogenesis Through MAP Kinase-Mediated Phosphorylation of PPARγ
Hu, E., Kim, J. B., Sarraf, P., & Spiegelman, B. M. (1996) · Science · doi:10.1126/science.274.5295.2100 · Cited by 1,050
The MEK/ERK cascade: From signaling specificity to diverse functions
Shaul, Y. D., & Seger, R. (2007) · Biochimica et Biophysica Acta (BBA) - Molecular Cell Research · doi:10.1016/j.bbamcr.2006.10.005 · Cited by 940
Metadata
- Authors
- Gonzalez, F. A., Raden, D. L., & Davis, R. J.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1991-11
- Volume
- 266
- Issue
- 33
- Pages
- 22159-22163
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1507957953
- Cited by
- 446
- References
- 0
- FWCI
- 9.26
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Gonzalez, F. A., Raden, D. L., & Davis, R. J. (1991). Identification of substrate recognition determinants for human ERK1 and ERK2 protein kinases. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(18)54548-8
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