The DOI matches its Crossref record; 8 of the 8 references it cites were found in a registry or an index.
Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes.
Open accessAbstract
Small amounts (7-250 pmol) of myoglobin, beta-lactoglobulin, and other proteins and peptides can be spotted or electroblotted onto polyvinylidene difluoride (PVDF) membranes, stained with Coomassie Blue, and sequenced directly. The membranes are not chemically activated or pretreated with Polybrene before usage. The average repetitive yields and initial coupling of proteins spotted or blotted into PVDF membranes ranged between 84-98% and 30-108% respectively, and were comparable with the yields measured for proteins spotted onto Polybrene-coated glass fiber discs. The results suggest that PVDF membranes are superior supports for sequence analysis of picomole quantities of proteins purified by gel electrophoresis.
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Cites
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Self-citationPolyquarternary amines prevent peptide loss from sequenators
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Cited by
Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programed cell death
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Metadata
- Authors
- Matsudaira, P.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1987-07
- Volume
- 262
- Issue
- 21
- Pages
- 10035-10038
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1932052686
- Cited by
- 4,950
- References
- 8
- FWCI
- 220.23
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Matsudaira, P. (1987). Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(18)61070-1
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
