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Reversible Denaturation of Enzymes by Sodium Dodecyl Sulfate
Open accessAbstract
Successful renaturation of several oligomeric enzymes after denaturation by sodium dodecyl sulfate is described. The results obtained suggest a general procedure for reactivation of enzymes after treatment with this detergent. Proteins in dodecyl sulfate solution are first incubated in concentrated urea and are then freed of the detergent by an anion exchange resin. The resulting dodecyl sulfate-free proteins can be renatured from urea solution by standard procedures. This method has also been used to recover enzymatic activity in moderate yields of some proteins after electrophoresis on polyacrylamide gels in the presence of sodium dodecyl sulfate.
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Cited by
Purification of a new neurotrophic factor from mammalian brain.
Barde, Y. A., Edgar, D., & Thoenen, H. (1982) · The EMBO Journal · doi:10.1002/j.1460-2075.1982.tb01207.x · Cited by 1,822
The size and detergent binding of membrane proteins.
Clarke, S. (1975) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)41204-0 · Cited by 451
Shikita, M., & Hall, P. F. (1973) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)43546-1 · Cited by 154
Shikita, M., & Hall, P. F. (1973) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)43547-3 · Cited by 61
The benefits (and misfortunes) of SDS in top-down proteomics
Kachuk, C., & Doucette, A. A. (2018) · Journal of Proteomics · doi:10.1016/j.jprot.2017.03.002 · Cited by 39
Metadata
- Authors
- Weber, K., & Kuter, D. J.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1971-07
- Volume
- 246
- Issue
- 14
- Pages
- 4504-4509
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1579567108
- Cited by
- 437
- References
- 0
- FWCI
- 13.35
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Weber, K., & Kuter, D. J. (1971). Reversible Denaturation of Enzymes by Sodium Dodecyl Sulfate. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(18)62040-x
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