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Further Characterization of Bovine Superoxide Dismutase and Its Isolation from Bovine Heart

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Keele, Bernard B., McCord, J.M., Fridovich, I.·1971·Journal of Biological Chemistry

Abstract

Amino acid analysis, electron resonance spectroscopy, metal analysis, and analysis for subunit structure have been performed on bovine superoxide dismutase. The enzyme consists of two subunits of identical molecular weight held together by disulfide bonds. Superoxide dismutase has been purified from bovine heart by a procedure which was previously developed for the isolation of this enzyme from bovine erythrocytes. The heart enzyme is judged to be identical with the erythrocyte enzyme on the basis of response to an unusual purification procedure, specific enzymatic activity, content of copper and zinc, molecular weight, amino acid composition, ultraviolet absorption spectrum, and electron paramagnetic resonance spectrum. The widespread distribution of superoxide dismutase in mammalian tissues is taken as one indication of its importance to the survival of the cells which constitute these diverse tissues.

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Metadata

Authors
Keele, B. B., McCord, J. M., & Fridovich, I.
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
1971-05
Volume
246
Issue
9
Pages
2875-2880
ISSN
0021-9258 (ISSN-L)
OpenAlex
W1659903845
Cited by
203
References
0
FWCI
9.43
Type
journal article
Language
en
Open access
hybrid

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CoChat. (2026). Citation check: Further Characterization of Bovine Superoxide Dismutase and Its Isolation from Bovine Heart. https://app.cochat.ai/papers/10.1016/s0021-9258(18)62263-x
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Keele, B. B., McCord, J. M., & Fridovich, I. (1971). Further Characterization of Bovine Superoxide Dismutase and Its Isolation from Bovine Heart. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(18)62263-x

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.