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The Sialic Acids
Open accessAbstract
The Svennerholm resorcinol method for the quantitative determination of the sialic acids was modified by introducing a periodate oxidation step prior to heating with the resorcinol reagent. Sialic acid glycosides could be determined without acid hydrolysis. The glycosides gave chromogens stable to periodate oxidation at 37°, whereas free sialic acid gave chromogens that were destroyed at this temperature, but were stable at 0°. Using these properties, the periodate-resorcinol method was applied to the determination of total, free, or bound sialic acid. The periodate-resorcinol method was substantially more sensitive than the resorcinol procedure, was not affected by lipids, amino acids, or sugars, and could be used to detect free or glycosidically bound sialic acids on paper chromatograms.
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Cited by
Fisher, L. W., Hawkins, G. R., Tuross, N., & Termine, J. D. (1987) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)47991-4 · Cited by 441
Kafetzopoulos, D., Martinou, A., & Bouriotis, V (1993) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.90.7.2564 · Cited by 254
Schüpbach, P., Neeser, J. R., Golliard, M., Rouvet, M., & Guggenheim, B. (1996) · Journal of Dental Research · doi:10.1177/00220345960750101101 · Cited by 126
The lubricating activity of human synovial fluids
Swann, D. A., Bloch, K. J., Swindell, D., & Shore, E. (1984) · Arthritis & Rheumatism · doi:10.1002/art.1780270511 · Cited by 54
Baky, N. A. A., Zaidi, Z. F., Fatani, A. J., Sayed-Ahmed, M. M., & Yaqub, H. (2010) · Brain Research Bulletin · doi:10.1016/j.brainresbull.2010.07.004 · Cited by 41
Metadata
- Authors
- Jourdian, G. W., Dean, L., & Roseman, S.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1971-01
- Volume
- 246
- Issue
- 2
- Pages
- 430-435
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1589482850
- Cited by
- 1,003
- References
- 0
- FWCI
- 8.44
- Type
- journal article
- Language
- en
- Open access
- hybrid
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
