The DOI matches its Crossref record; 11 of the 11 references it cites were found in a registry or an index.
Bioconversion of chitin to chitosan: purification and characterization of chitin deacetylase from Mucor rouxii.
Open accessAbstract
Chitin deacetylase, the enzyme that catalyzes the hydrolysis of acetamido groups of N-acetylglucosamine in chitin, has been purified to homogeneity from mycelial extracts of the fungus Mucor rouxii and further characterized. The enzyme exhibits a low pI (approximately 3). Its apparent molecular mass was determined to be approximately 75 kDa by sodium dodecyl sulfate/polyacrylamide gel electrophoresis and approximately 80 kDa by size-exclusion chromatography, suggesting that the enzyme exists as a monomer. Carbohydrate analysis of purified chitin deacetylase revealed that the enzyme is a high-mannose glycoprotein and that its carbohydrate content is approximately 30% by weight. Chitin deacetylase is active on several chitinous substrates and chitin derivatives. The enzyme requires at least four N-acetylglucosamine residues (chitotetraose) for catalysis, and it is inhibited by carboxylic acids, particularly acetic acid. When glycol chitin (a water-soluble chitin derivative) was used as substrate, the optimum temperature for enzyme activity was determined to be approximately 50 degrees C and the optimum pH was approximately 4.5.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
LAEMMLI, U. K. (1970) · Nature · doi:10.1038/227680a0 · Cited by 249,961
Raza, H., John, A., & Nemmar, A. (2013) · Cellular Physiology and Biochemistry · doi:10.1159/000350087 · Cited by 47,221
DISC ELECTROPHORESIS – II METHOD AND APPLICATION TO HUMAN SERUM PROTEINS*
Davis, B. J. (1964) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.1964.tb14213.x · Cited by 18,892
A simplification of the protein assay method of Lowry et al. which is more generally applicable
Peterson, G. L. (1977) · Analytical Biochemistry · doi:10.1016/0003-2697(77)90043-4 · Cited by 8,524
Sawardeker, J. S., Sloneker, J. H., & Jeanes, A. (1965) · Analytical Chemistry · doi:10.1021/ac60231a048 · Cited by 1,701
Ultraviolet Absorption Spectra of Proteins and Amino Acids
Beaven, G. H., & Holiday, E. R. (1952) · Advances in Protein Chemistry · doi:10.1016/s0065-3233(08)60022-4 · Cited by 1,113
Jourdian, G. W., Dean, L., & Roseman, S. (1971) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)62508-6 · Cited by 1,003
Bartnicki-Garcia, S., & Nickerson, W. J. (1962) · Biochimica et Biophysica Acta · doi:10.1016/0006-3002(62)90822-3 · Cited by 358
A Pathway of Chitosan Formation in Mucor rouxii: Enzymatic Deacetylation of Chitin
ARAKI, Y., & ITO, E. (1975) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1975.tb02139.x · Cited by 233
The Synthesis and Degradation of Chitin
Cabib, E. (1987) · Advances in Enzymology - and Related Areas of Molecular Biology · doi:10.1002/9780470123058.ch2 · Cited by 180
Cited by
Chitosan: an update on potential biomedical and pharmaceutical applications.
Cheung, R., Ng, T., Wong, J., & Chan, W. (2015) · Marine Drugs · doi:10.3390/md13085156 · Cited by 1,297
Multiple effects of chitosan on plant systems: Solid science or hype
Hadwiger, L. A. (2013) · Plant Science · doi:10.1016/j.plantsci.2013.03.007 · Cited by 358
Vander, P., Vårum, K. M., Domard, A., Eddine El Gueddari, N., & Moerschbacher, B. M. (1998) · Plant Physiology · doi:10.1104/pp.118.4.1353 · Cited by 339
Banks, I. R., Specht, C. A., Donlin, M. J., Gerik, K. J., Levitz, S. M., & Lodge, J. K. (2005) · Eukaryotic Cell · doi:10.1128/ec.4.11.1902-1912.2005 · Cited by 245
Metadata
- Authors
- Kafetzopoulos, D., Martinou, A., & Bouriotis, V
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1993-04
- Volume
- 90
- Issue
- 7
- Pages
- 2564-2568
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1995395488
- Cited by
- 254
- References
- 11
- FWCI
- 1.37
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Bioconversion of chitin to chitosan: purification and characterization of chitin deacetylase from Mucor rouxii. https://app.cochat.ai/papers/10.1073/pnas.90.7.2564
- The paper
- Kafetzopoulos, D., Martinou, A., & Bouriotis, V. (1993). Bioconversion of chitin to chitosan: purification and characterization of chitin deacetylase from Mucor rouxii. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.90.7.2564
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
