The DOI matches its Crossref record; 19 of the 19 references it cites were found in a registry or an index.
Identification of a Lipid A Binding Site in the Acute Phase Reactant Lipopolysaccharide Binding Protein
Open accessAbstract
Lipopolysaccharide (LPS) binding protein (LBP), a recently discovered 60-kDa acute phase protein, is present in the acute phase serum of many species including human, rabbits, mice, and rats. Using either highly purified LBP from acute phase rabbit serum or unfractionated acute phase rabbit serum as a source of LBP, we examined the binding of LBP to LPS immobilized on plastic microtiter plates and to LPS electrotransferred to nitrocellulose after sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The presence of LBP bound to LPS was detected with goat anti-rabbit LBP and peroxidase-conjugated rabbit anti-goat IgG. LBP was found to bind to a variety of LPS types from both rough and smooth strains of Gram-negative bacteria, to lipid A, and to the tetraacyl glucosamine disaccharide diphosphate precursor IVA, but bound very poorly to the diacyl glucosamine phosphate, lipid X. No binding to 3-deoxyoctulosonic acid was observed. Binding affinities for LPS are near 10(9) M-1. The data presented here support the concept that LBP contains a binding site for lipid A.
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Cites
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Self-citationElectrodialysis of Lipopolysaccharides and Their Conversion to Uniform Salt Forms
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Cited by
Defective LPS Signaling in C3H/HeJ and C57BL/10ScCr Mice: Mutations in Tlr4 Gene
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Agents that increase the permeability of the outer membrane
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The immune response of Drosophila
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Metadata
- Authors
- Tobias, P. S., Soldau, K., & Ulevitch, R. J.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1989-06
- Volume
- 264
- Issue
- 18
- Pages
- 10867-10871
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1582984670
- Cited by
- 347
- References
- 19
- FWCI
- 4.76
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Tobias, P. S., Soldau, K., & Ulevitch, R. J. (1989). Identification of a Lipid A Binding Site in the Acute Phase Reactant Lipopolysaccharide Binding Protein. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(18)81700-8
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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