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Identification of the cystines which link the acidic and basic components of the glycinin subunits.
Open accessAbstract
The half-cystine residues involved in linking the acidic and basic polypeptides were determined for several glycinin subunits. The cystines were localized with specific cyanogen bromide fragments either by comparing the electrophoretic mobility of nonreduced and reduced fragments, or by co-purifying and then determining the NH2-terminal sequence of the covalently linked fragments. Residues involved in the disulfides were further identified by labeling them with [3H] iodoacetic acid. Only 1 cystine was found to be involved in linking the acidic and basic components of each subunit, and they were in analogous positions in each of the subunits studied. Potential sites for intrapolypeptide cystines were also identified.
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Cited by
Nonmeat Protein Alternatives as Meat Extenders and Meat Analogs
Asgar, M. A., Fazilah, A., Huda, N., Bhat, R., & Karim, A. A. (2010) · Comprehensive Reviews in Food Science and Food Safety · doi:10.1111/j.1541-4337.2010.00124.x · Cited by 505
Characterization of the glycinin gene family in soybean.
Nielsen, N. C., Dickinson, C. D., Cho, T. J., Thanh, V. H., Scallon, B. J., Fischer, R. L., Sims, T. L., Drews, G. N., & Goldberg, R. B. (1989) · The Plant Cell · doi:10.1105/tpc.1.3.313 · Cited by 382
Self-citationDeposition of storage proteins
Müntz, K. (1998) · Plant Molecular Biology · doi:10.1023/a:1006020208380 · Cited by 280
Functional Properties of Improved Glycinin and β-nglycinin Fractions
Rickert, D. A., Johnson, L. A., & Murphy, P. A. (2004) · Journal of Food Science · doi:10.1111/j.1365-2621.2004.tb06332.x · Cited by 116
Metadata
- Authors
- Staswick, P. E., Hermodson, M. A., & Nielsen, N. C.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1984-11
- Volume
- 259
- Issue
- 21
- Pages
- 13431-13435
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1549946077
- Cited by
- 200
- References
- 0
- FWCI
- 2.01
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Staswick, P. E., Hermodson, M. A., & Nielsen, N. C. (1984). Identification of the cystines which link the acidic and basic components of the glycinin subunits. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(18)90712-x
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
