The DOI matches its Crossref record; 8 of the 8 references it cites were found in a registry or an index.
Purification and identification of formyl-methionyl-leucyl-phenylalanine as the major peptide neutrophil chemotactic factor produced by Escherichia coli.
Open accessAbstract
Chemotactic factor-enriched butanol extracts from Escherichia coli culture filtrates were fractionated and purified by high pressure liquid chromatography. The yield from individual fractions of biological activity (lysosomal enzyme secretion) and antigenic activity (competition with [3H]fMet-Leu-Phe for binding to rabbit anti-fMet-Leu-Phe) revealed an average 50% recovery of original material. Five peaks of biological activity were separated as demonstrated by enzyme-releasing activity. Three of these peaks coincided exactly with peaks of antigenic activity, suggesting that at least 3 and as many as 5 distinct formyl-methionyl peptides had been separated. The majority of recovered activity appeared in peak 3 and represented 70% of the total biological and antigenic activities recovered. The five peak fractions were subsequently analyzed by dipeptidyl carboxypeptidase gas chromatography-mass spectrometry (DCP/GC-MS) to determine amino acid sequences. After digestion, the formyl-Met peptide was demonstrated in only one of the five peak fractions (peak 3). Furthermore, both the GC retention times and mass spectra indicated that peak 3 contained formyl-methionyl-leucyl-phenylalanine. The DCP/GC and MS data were confirmed with tests made on authentic fMet-Leu-Phe. Butanol extracts from E. coli filtrates to which were added synthetic fMet-Leu-Phe resulted in increased biological and antigenic activity in the precise high pressure liquid chromatography fractions of peak 3 where the fMet-Leu-Phe produced by E. coli was found. Finally, the analysis of recovered biological and antigenic activities indicated that the formyl peptides were found in nanomolar concentrations in culture filtrates. These results demonstrate that the NH2-terminal formyl peptides produced by E. coli, of which formyl-methionyl-leucyl-phenylalanine appears to be the major component, are the peptide mediators responsible for leukocyte chemotactic activity in the bacterial culture extracts.
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Cites
Perlman, D., & Halvorson, H. O. (1983) · Journal of Molecular Biology · doi:10.1016/s0022-2836(83)80341-6 · Cited by 1,131
N-formylmethionyl peptides as chemoattractants for leucocytes.
Schiffmann, E., Corcoran, B. A., & Wahl, S. M. (1975) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.72.3.1059 · Cited by 881
Showell, H. J., Freer, R. J., Zigmond, S. H., Schiffmann, E., Aswanikumar, S., Corcoran, B., & Becker, E. L. (1976) · The Journal of experimental medicine · doi:10.1084/jem.143.5.1154 · Cited by 735
Self-citationSpecific receptor sites for chemotactic peptides on human polymorphonuclear leukocytes.
Williams, L. T., Snyderman, R., Pike, M. C., & Lefkowitz, R. J. (1977) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.74.3.1204 · Cited by 541
Bacterial factors chemotactic for polymorphonuclear leukocytes.
Ward, P. A., Lepow, I. H., & Newman, L. J. (1968) · PubMed · Cited by 297
Self-citationThe Isolation and Partial Characterization of Neutrophil Chemotactic Factors from Escherichia Coli
Schiffmann, E., Showell, H. V., Corcoran, B. A., Ward, P. A., Smith, E., & Becker, E. L. (1975) · The Journal of Immunology · doi:10.4049/jimmunol.114.6.1831 · Cited by 269
Self-citationFurther studies on the structural requirements for synthetic peptide chemoattractants
Freer, R. J., Day, A. R., Radding, J. A., Schiffmann, E., Aswanikumar, S., Showell, H. J., & Becker, E. L. (1980) · Biochemistry · doi:10.1021/bi00552a019 · Cited by 226
Self-citationMarasco, W. A., Fantone, J. C., & Ward, P. A. (1982) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.79.23.7470 · Cited by 20
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Cited by
The Immunology of Mucosal Models of Inflammation
Strober, W., Fuss, I. J., & Blumberg, R. S. (2002) · Annual Review of Immunology · doi:10.1146/annurev.immunol.20.100301.064816 · Cited by 1,304
Macrophages and Polymorphonuclear Neutrophils in Lung Defense and Injury
Sibille, Y., & Reynolds, H. Y. (1990) · American Review of Respiratory Disease · doi:10.1164/ajrccm/141.2.471 · Cited by 1,083
Xue, J., Zhao, Z., Zhang, L., Xue, L., Shen, S., Wen, Y., Wei, Z., Wang, L., Kong, L., Sun, H., et al. (2017) · Nature Nanotechnology · doi:10.1038/nnano.2017.54 · Cited by 959
hPepT1 mediates bacterial tripeptide fMLP uptake in human monocytes
Charrier, L., Driss, A., Yan, Y., Nduati, V., Klapproth, J.-M., Sitaraman, S. V., & Merlin, D. (2006) · Laboratory Investigation · doi:10.1038/labinvest.3700413 · Cited by 52
Inhibition of formyl peptide receptors improves the outcome in a mouse model of Alzheimer disease
Schröder, N., Schaffrath, A., Welter, J. A., Putzka, T., Griep, A., Ziegler, P., Brandt, E., Samer, S., Heneka, M. T., Kaddatz, H., et al. (2020) · Journal of Neuroinflammation · doi:10.1186/s12974-020-01816-2 · Cited by 43
Metadata
- Authors
- Marasco, W. A., Phan, S. H., Krutzsch, H., Showell, H. J., Feltner, D. E., Nairn, R., Becker, E. L., & Ward, P. A.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1984-05
- Volume
- 259
- Issue
- 9
- Pages
- 5430-5439
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1531938298
- Cited by
- 521
- References
- 8
- FWCI
- 8.76
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- CoChat. (2026). Citation check: Purification and identification of formyl-methionyl-leucyl-phenylalanine as the major peptide neutrophil chemotactic factor produced by Escherichia coli. https://app.cochat.ai/papers/10.1016/s0021-9258(18)91029-x
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- Marasco, W. A., Phan, S. H., Krutzsch, H., Showell, H. J., Feltner, D. E., Nairn, R., Becker, E. L., & Ward, P. A. (1984). Purification and identification of formyl-methionyl-leucyl-phenylalanine as the major peptide neutrophil chemotactic factor produced by Escherichia coli. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(18)91029-x
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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