The DOI matches its Crossref record.
The Biosynthesis of Cell Wall Lipopolysaccharide in Escherichia coli
Open accessAbstract
Abstract An acidic component of the cell wall lipopolysaccharide of Escherichia coli 0111-B4 was isolated from mild acid hydrolysates of the polymer. It was concluded that this compound was 3-deoxy-D-manno-octulosonic acid on the basis of the following criteria: (a) paper chromatography in a variety of solvent systems; (b) degradation with ceric sulfate following borohydride reduction; (c) enzymatic degradation by 3-deoxyoctulosonate (KDO) aldolase to yield pyruvate and D-arabinose; (d) the rate of liberation of β-formyl pyruvate by periodate oxidation; (e) chemical synthesis of crystalline penta-O-acetyl-KDO methyl ester, which, upon alkaline saponification, yielded KDO which corresponded chromatographically to KDO from lipopolysaccharide. Further, the product of saponification was identical with natural KDO with regard to the specificities of cytidine 5'-phosphate-KDO synthetase and KDO aldolase. The procedure employed for the chemical synthesis of KDO provides a method for the preparation of a chemically pure standard of this compound.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
Protection against gram-negative bacteremia and endotoxemia with human monoclonal IgM antibodies.
Teng, N. N., Kaplan, H. S., Hebert, J. M., Moore, C., Douglas, H., Wunderlich, A., & Braude, A. I. (1985) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.82.6.1790 · Cited by 313
Prevention of clinical coliform mastitis in dairy cows by a mutant Escherichia coli vaccine.
(1989) · Europe PMC (PubMed Central) · Cited by 109
Field trial to determine efficacy of an Escherichia coli J5 mastitis vaccine
Hogan, J. S., Smith, K. L., Todhunter, D. A., & Schoenberger, P. S. (1992) · Journal of Dairy Science · doi:10.3168/jds.s0022-0302(92)77741-8 · Cited by 96
Immunity targeting common core antigens of Gram-negative bacteria
Tyler, J. W., Cullor, J. S., Spier, S. J., & Smith, B. P. (1990) · Journal of Veterinary Internal Medicine · doi:10.1111/j.1939-1676.1990.tb00870.x · Cited by 33
Opsonic Activity of Bovine Serum and Mammary Secretion After Escherichia coli J5 Vaccination
Hogan, J. S., Todhunter, D. A., Tomita, G. M., Smith, K. L., & Schoenberger, P. S. (1992) · Journal of Dairy Science · doi:10.3168/jds.s0022-0302(92)77740-6 · Cited by 22
Metadata
- Authors
- Elbein, A. D., & Heath, E. C.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1965-05
- Volume
- 240
- Issue
- 5
- Pages
- 1919-1925
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W41260302
- Cited by
- 163
- References
- 0
- FWCI
- 31.72
- Type
- journal article
- Language
- en
- Open access
- hybrid
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: The Biosynthesis of Cell Wall Lipopolysaccharide in Escherichia coli. https://app.cochat.ai/papers/10.1016/s0021-9258(18)97405-3
- The paper
- Elbein, A. D., & Heath, E. C. (1965). The Biosynthesis of Cell Wall Lipopolysaccharide in Escherichia coli. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(18)97405-3
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
