Activation of Na+ and K+ pumping modes of (Na, K)-ATPase by an oscillating electric field
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Serpersu and Tsong (Sepersu, E. H., and Tsong, T. Y. (1983) J. Membr. Biol. 74, 191-201; (1984) J. Biol. Chem. 259, 7155-7162) reported activation of a K+ pumping mode of (Na,K)-ATPase by an oscillating electric field (20 V/cm, 1.0 kHz). Their attempts to activate Na+ pumping at the same frequency were unsuccessful. We report here activation of a Na+ pumping mode with an oscillating electric field of the same strength as used previously (20 V/cm) but at a much higher frequency (1.0 MHz). At 3.5 degrees C and the optimal amplitude and frequency, the field-induced, ouabain-sensitive (0.2 mM ouabain incubated for 30 min) Rb+ influx ranged between 10 and 20 amol/red blood cell/h, and the corresponding Na+ efflux ranged between 15 and 30 amol/red blood cell/h, varying with the source of the erythrocytes. No Rb+ efflux nor Na+ influx was stimulated by the applied field in the frequency range 1 Hz to 10 MHz. These results indicate that only those transport modes that require ATP splitting under the physiological condition were affected by the applied electric fields, although the field-stimulated Rb+ influx and Na+ efflux did not depend on the cellular ATP concentration in the range 5 to 800 microM. Computer simulation of a four-state enzyme electroconformationally coupled to an alternating electric field (Tsong, T. Y., and Astumian, R. D. (1986) Bioelectrochem. Bioenerg. 15, 457-476; Tsong, T. Y. (1990) Annu. Rev. Biophys. Biophys. Chem. 19, 83-106) reproduced the main features of the above results.
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Neither K+ efflux nor Na+ influx was stimulated by the applied field in the frequency range from 1 Hz to 10 MHz.
“Moreover, neither K+ efflux nor Na+ influx was stimulated by the applied field in the frequency range from 1 Hz to 10 MHz.”
From An Overview of Sub-Cellular Mechanisms Involved in the Action of TTFields (2016) · cites this paper as [80]
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Astumian, R. D. (1996) · The Journal of Physical Chemistry · doi:10.1021/jp961614m · Cited by 39 · see citing works
The stochastic pump current and the non-adiabatic geometrical phase
Ohkubo, J. (2008) · Journal of Statistical Mechanics Theory and Experiment · doi:10.1088/1742-5468/2008/02/p02011 · Cited by 38 · see citing works
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Woodward, A. M., Jones, A., Zhang, X.-Z., Rowland, J. J., & Kell, D. B. (1996) · Bioelectrochemistry and Bioenergetics · doi:10.1016/0302-4598(96)05065-9 · Cited by 38 · see citing works
Ion activation of the Na,K-ATPase in alternating currents
Blank, M., & Soo, L. (1990) · Bioelectrochemistry and Bioenergetics · doi:10.1016/0302-4598(80)85006-9 · Cited by 38 · see citing works
The Bioelectric Circuitry of the Cell
Tuszyński, J. A. (2019) · Springer eBooks · doi:10.1007/978-3-030-21293-3_11 · Cited by 37 · see citing works
Woodward, A. M., & Kell, D. B. (1991) · Bioelectrochemistry and Bioenergetics · doi:10.1016/0302-4598(91)85005-m · Cited by 34 · see citing works
Kosińska, I. D., & Fuliński, A. (2005) · Physical Review E · doi:10.1103/physreve.72.011201 · Cited by 33 · see citing works
Weaver, J. C., & Astumian, R. D. (1992) · Bioelectromagnetics · doi:10.1002/bem.2250130712 · Cited by 32 · see citing works
Composition-driven Surface Domain Structuring Mediated by Sphingolipids and Membrane-active Proteins
Maggio, B., Borioli, G. A., Boca, M. D., Tullio, L. D., Fanani, M. L., Oliveira, R. G., Rosetti, C. M., & Wilke, N. (2007) · Cell Biochemistry and Biophysics · doi:10.1007/s12013-007-9004-1 · Cited by 30 · see citing works
Current and fluctuation in a two-state stochastic system under nonadiabatic periodic perturbation
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Activated escape over oscillating barriers: The case of many dimensions
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Cell fission and formation of mini cell bodies by high frequency alternating electric field
Marszałek, P. E., & Tsong, T. Y. (1995) · Biophysical Journal · doi:10.1016/s0006-3495(95)80338-3 · Cited by 30 · see citing works
Interpretation of the effect of an oscillating electric field on membrane enzymes
Robertson, B., & Astumian, R. D. (1992) · Biochemistry · doi:10.1021/bi00116a020 · Cited by 30 · see citing works
Universal Character of Stochastic Resonance and a Constructive Role of White Noise
Fuliński, A., & Góra, P. (2000) · Journal of Statistical Physics · doi:10.1023/a:1026463523619 · Cited by 29 · see citing works
Lahijani, M. S., Tehrani, D. M., & SABOURI, E. (2009) · Electromagnetic Biology and Medicine · doi:10.3109/15368370903287689 · Cited by 28 · see citing works
“…Greenebaum et al., 1991; Grooten et al., 2007; Guler et al., 2007; Hannig and Lee, 2000; Harakawa et al., 2005; Hook et al., 2004; Ivancsits et al., 2003; Liu et al., 1990; Masazumi et al., 1999; McCaig, 1997; Mishchenko, 1969; Miyakushi, 2006; Miyakushi et al., 2000; Morehouse and Owen, 2000;…”
BackgroundConstructive role of noise in signal transmissions by biomembrane proteins
Fuliński, A., & Góra, P. (2001) · Physical review. E, Statistical physics, plasmas, fluids, and related interdisciplinary topics · doi:10.1103/physreve.64.011905 · Cited by 28 · see citing works
Connections between the Specific Absorption Rate and the Local Temperature
Szász, O., Szigeti, G. P., & Szász, A. (2016) · Open Journal of Biophysics · doi:10.4236/ojbiphy.2016.63007 · Cited by 27 · see citing works
“The electric field promotes cellular fusion at low [89] and high [90] frequencies; a field-strength-dependent haemolytic effect was observed by RF exposure [91], activation of ion-channels at the cellular membrane [92], membrane mediated Ca2+ signalling affects the immune system, [93], and transmembrane Ca2+ was induc…”
BackgroundThe Na,K-ATPase as a model for electromagnetic field effects on cells
Blank, M., & Soo, L. (1993) · Bioelectrochemistry and Bioenergetics · doi:10.1016/0302-4598(93)80065-3 · Cited by 27 · see citing works
Effects of a low-voltage static electric field on energy metabolism in astrocytes
Huang, R., Peng, L., & Hertz, L. (1997) · Bioelectromagnetics · doi:10.1002/(sici)1521-186x(1997)18:1 · Cited by 27 · see citing works
Barrier fluctuations and stochastic resonance in membrane transport
Fuliński, A. (1998) · Chaos An Interdisciplinary Journal of Nonlinear Science · doi:10.1063/1.166336 · Cited by 26 · see citing works
Aksyonov, S. I., Bulychev, A. A., Грунина, Т. Ю., Goryachev, S. N., & Turovetsky, V. B. (2001) · Electro- and Magnetobiology · doi:10.1081/jbc-100104146 · Cited by 25 · see citing works
Electromagnetic window effects on proliferation rate of Corynebacterium glutamicum
Lei, C., & Berg, H. (1998) · Bioelectrochemistry and Bioenergetics · doi:10.1016/s0302-4598(98)00099-3 · Cited by 24 · see citing works
On electroporation of cell membranes and some related phenomena
Tsong, T. Y. (1990) · Journal of Electroanalytical Chemistry · doi:10.1016/0022-0728(90)87529-s · Cited by 24 · see citing works
Time Sequence of Molecular Events in Electroporation
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Noise-stimulated active transport in biological cell membranes
Fuliński, A. (1994) · Physics Letters A · doi:10.1016/0375-9601(94)90595-9 · Cited by 24 · see citing works
The role of thermal activation in motion and force generation by molecular motors
Astumian, R. D. (2000) · Philosophical Transactions of the Royal Society B Biological Sciences · doi:10.1098/rstb.2000.0592 · Cited by 23 · see citing works
“The rigid requirements for such clock-like coupling have recently been challenged by experiments of Tsong and colleagues (Liu et al. 1990; Xie et al. 1994) on Na,KATPase.”
BackgroundMethodMicrobial decontamination of food by pulsed electric fields (PEFs)
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Electrically Stimulated Membrane Breakdown
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Ion channel enzyme in an oscillating electric field
Markin, V., Liu, D., Gimsa, J., Strobel, R. S., Rosenberg, M. D., & Tsong, T. Y. (1992) · The Journal of Membrane Biology · doi:10.1007/bf00231912 · Cited by 22 · see citing works
Tsong, T. Y. (2002) · Journal of Biological Physics · doi:10.1023/a:1019991918315 · Cited by 21 · see citing works
Reversible mechanosensitive ion pumping as a part of mechanoelectrical transduction
Markin, V. S., & Tsong, T. Y. (1991) · Biophysical Journal · doi:10.1016/s0006-3495(91)82346-3 · Cited by 21 · see citing works
“In the preceding investigations, an electroconformational coupling (ECC) model for membrane transport was developed which could transduce the energy of an oscillating electrical field into the concentration gradient of a ligand (1-12).”
BackgroundMarkin, V. S., & Tsong, T. Y. (1991) · Journal of Electroanalytical Chemistry · doi:10.1016/0022-0728(91)85600-t · Cited by 21 · see citing works
On the nonlinear dielectric properties of biological systems
Woodward, A. M., & Kell, D. B. (1990) · Journal of Electroanalytical Chemistry · doi:10.1016/0022-0728(90)87511-h · Cited by 20 · see citing works
Wilke, N., & Maggio, B. (2006) · Biophysical Chemistry · doi:10.1016/j.bpc.2006.02.008 · Cited by 19 · see citing works
Electrowetting of Weak Polyelectrolyte-Coated Surfaces
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Interaction of low level modulated RF radiation with Na+–K+-ATPase
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- Authors
- Liu, D. S., Astumian, R. D., & Tsong, T. Y.
- Affiliations
- University of Minnesota (US)
- ORCID
- Astumian, R D
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1990-05
- Volume
- 265
- Issue
- 13
- Pages
- 7260-7267
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1608844245
- Cited by
- 199
- References
- 43
- Citations by year
- 2026: 2 · 2024: 4 · 2023: 1 · 2022: 3 · 2021: 4 · 2020: 6 · 2019: 4 · 2018: 3 · 2017: 3 · 2016: 10 · 2015: 2 · 2012: 4
- FWCI
- 5.84
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion Transport and Channel Regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › ATP Synthase and ATPases Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Electromagnetic Fields and Biological Effects
- Keywords
- Na+/K+-ATPase, oscillating electric field, Na+ efflux, erythrocytes, ouabain sensitivity, ATP hydrolysis, erythrocyte membrane
- MeSH terms
- Sodium-Potassium-Exchanging ATPase, Adenosine Triphosphate, Biological Transport, Active, Electric Stimulation, Erythrocytes, Humans, Kinetics, Mathematics, Models, Theoretical, Ouabain, Potassium, Rubidium, Sodium, Spectrometry, Fluorescence
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