The DOI matches its Crossref record; 29 of the 29 references it cites were found in a registry or an index.
Purification of the 170- to 180-kilodalton membrane glycoprotein associated with multidrug resistance. 170- to 180-kilodalton membrane glycoprotein is an ATPase.
Open accessAbstract
170-180-kDa membrane glycoprotein (P-glycoprotein) associated with multidrug resistance is involved in drug transport mechanisms across the plasma membrane of resistant cells. From sequence analysis of cDNAs of the P-glycoprotein gene, it is postulated that the active drug-efflux pump function may be attributable to the protein. However, purification of the P-glycoprotein while preserving its enzymatic activity has not been reported. In this study, we have purified the P-glycoprotein from the human myelogenous leukemia K562 cell line resistant to adriamycin (K562/ADM) by means of one-step immunoaffinity chromatography using a monoclonal antibody against P-glycoprotein. The procedure was simple and efficiently yielded an electrophoretically homogeneous P-glycoprotein sample. By solubilization with 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate, the purified P-glycoprotein was found to have ATPase activity. This ATP hydrolysis may be coupled with the active efflux of anticancer drugs across the plasma membrane of multidrug-resistant cells.
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Higgins, C. F., & Gottesman, M. M. (1992) · Trends in Biochemical Sciences · doi:10.1016/0968-0004(92)90419-a · Cited by 722
Sarkadi, B., Price, E. M., Boucher, R. C., Germann, U. A., & Scarborough, G. A. (1992) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)42909-2 · Cited by 632
Kamijo, K., Taketani, S., Yokota, S., Osumi, T., & Hashimoto, T. (1990) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)39595-x · Cited by 391
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Metadata
- Authors
- Hamada, H., & Tsuruo, T.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1988-01
- Volume
- 263
- Issue
- 3
- Pages
- 1454-1458
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1531911812
- Cited by
- 332
- References
- 29
- FWCI
- 19.50
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Hamada, H., & Tsuruo, T. (1988). Purification of the 170- to 180-kilodalton membrane glycoprotein associated with multidrug resistance. 170- to 180-kilodalton membrane glycoprotein is an ATPase. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(19)57324-0
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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