The DOI matches its Crossref record.
Evidence for two functional gal promoters in intact Escherichia coli cells.
Open accessAbstract
We have used an S1 mapping assay to demonstrate that the mRNA transcripts of the Escherichia coli galactose operon found in intact E. coli cells with a defect in adenylate cyclase or the cyclic AMP receptor protein contain at their 5' end about five nucleotides more than the gal mRNA molecules made in wild type cells. The same difference between gal RNA synthesized in vitro in the absence of cyclic AMP or cyclic AMP receptor protein and gal RNA made in the presence of these factors is detected by this assay. Our results strongly suggest that the same two overlapping promoters, which we previously identified by in vitro transcription of gal DNA fragments, also control the expression of the galactose operon in intact cells. The intracellular levels of cyclic AMP determine which promoter is utilized.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
A small RNA regulates the expression of genes involved in iron metabolism in Escherichia coli
Massé, E., & Gottesman, S. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.032066599 · Cited by 1,154
Genes Essential to Iron Transport in the Cyanobacterium Synechocystis sp. Strain PCC 6803
Katoh, H., Hagino, N., Grossman, A. R., & Ogawa, T. (2001) · Journal of Bacteriology · doi:10.1128/jb.183.9.2779-2784.2001 · Cited by 204
A Family of S-Methylmethionine-dependent Thiol/Selenol Methyltransferases
Neuhierl, B., Thanbichler, M., Lottspeich, F., & Böck, A. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.9.5407 · Cited by 190
A Novel Porphyromonas gingivalis FeoB Plays a Role in Manganese Accumulation
Dashper, S. G., Butler, C. A., Lissel, J. P., Paolini, R. A., Hoffmann, B., Veith, P. D., O'Brien-Simpson, N. M., Snelgrove, S. L., Tsiros, J. T., & Reynolds, E. C. (2005) · Journal of Biological Chemistry · doi:10.1074/jbc.m503896200 · Cited by 90
Burger, M., Woods, R. G., McCarthy, C., & Beacham, I. R. (2000) · Microbiology · doi:10.1099/00221287-146-12-3149 · Cited by 48
Metadata
- Authors
- Aiba, H., Adhya, S., & de Crombrugghe, B.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1981-11
- Volume
- 256
- Issue
- 22
- Pages
- 11905-11910
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1562974039
- Cited by
- 1,022
- References
- 0
- FWCI
- 11.58
- Type
- journal article
- Language
- en
- Open access
- hybrid
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Evidence for two functional gal promoters in intact Escherichia coli cells. https://app.cochat.ai/papers/10.1016/s0021-9258(19)68491-7
- The paper
- Aiba, H., Adhya, S., & de Crombrugghe, B. (1981). Evidence for two functional gal promoters in intact Escherichia coli cells. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(19)68491-7
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
