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Cited by 1,022

The DOI matches its Crossref record.

Evidence for two functional gal promoters in intact Escherichia coli cells.

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Aiba, H., Adhya, S., de Crombrugghe, B.·1981·Journal of Biological Chemistry
DOI 10.1016/s0021-9258(19)68491-7
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Abstract

We have used an S1 mapping assay to demonstrate that the mRNA transcripts of the Escherichia coli galactose operon found in intact E. coli cells with a defect in adenylate cyclase or the cyclic AMP receptor protein contain at their 5' end about five nucleotides more than the gal mRNA molecules made in wild type cells. The same difference between gal RNA synthesized in vitro in the absence of cyclic AMP or cyclic AMP receptor protein and gal RNA made in the presence of these factors is detected by this assay. Our results strongly suggest that the same two overlapping promoters, which we previously identified by in vitro transcription of gal DNA fragments, also control the expression of the galactose operon in intact cells. The intracellular levels of cyclic AMP determine which promoter is utilized.

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Authors
Aiba, H., Adhya, S., & de Crombrugghe, B.
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
1981-11
Volume
256
Issue
22
Pages
11905-11910
ISSN
0021-9258 (ISSN-L)
DOI
10.1016/s0021-9258(19)68491-7
OpenAlex
W1562974039
Cited by
1,022
References
0
FWCI
11.58
Type
journal article
Language
en
Open access
hybrid

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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.

Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.