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Estimation of the percentage of free steroid in undiluted serum by centrifugal ultrafiltration-dialysis.
Open accessAbstract
A new method is described for the estimation of the percentages of free steroids in undiluted serum or plasma. Samples were incubated with 3H-steroid and [14C]glucose, and duplicate aliquots of the mixture were subjected to centrifugal ultrafiltration through a dialysis membrane at 37 degrees C. The percentages of free estradiol, testosterone, and progesterone in serum were estimated by comparing the ratio of 3H-steroid to [14C]glucose in the ultrafiltrate with the corresponding ratio in the serum retained by the dialysis membrane. In addition to being highly reproducible, the present method affords several advantages over existing methods. The use of [14C]glucose to monitor the movement of unbound serum components obviates measurement of the ultrafiltrate volume, and because the ultrafiltrate remains in contact with the dialysis membrane, small molecular weight components are able to pass through the dialysis membrane in either direction during centrifugal ultrafiltration. This latter feature, which could be described as "isodialysis," allows the estimation of the percentage of free steroid under conditions which closely approximate the in vivo situation.
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Citations
Cited by
DUNN, J. F., NISULA, B. C., & RODBARD, D. (1981) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem-53-1-58 · Cited by 1,238
The serum transport of steroid hormones
SIITERI, P. K., MURAI, J. T., RAYMOURE, W. J., KUHN, R. W., HAMMOND, G. L., & NISKER, J. A. (1982) · Proceedings of the 1981 Laurentian Hormone Conference · doi:10.1016/b978-0-12-571138-8.50016-0 · Cited by 770
Self-citationSalivary cortisol: a better measure of adrenal cortical function than serum cortisol
Vining, R. F., McGinley, R. A., Maksvytis, J. J., & Ho, K. Y. (1983) · Annals of Clinical Biochemistry International Journal of Laboratory Medicine · doi:10.1177/000456328302000601 · Cited by 684
Field, A. E., Colditz, G. A., Willett, W. C., Longcope, C., & McKinlay, J. B. (1994) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem.79.5.7962322 · Cited by 421
Plasma steroid-binding proteins: primary gatekeepers of steroid hormone action
Hammond, G. L. (2016) · Journal of Endocrinology · doi:10.1530/joe-16-0070 · Cited by 356
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Metadata
- Authors
- Hammond, G. L., Nisker, J. A., Jones, L. A., & Siiteri, P. K.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1980-06
- Volume
- 255
- Issue
- 11
- Pages
- 5023-5026
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1517007955
- Cited by
- 268
- References
- 0
- FWCI
- 6.76
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Hammond, G. L., Nisker, J. A., Jones, L. A., & Siiteri, P. K. (1980). Estimation of the percentage of free steroid in undiluted serum by centrifugal ultrafiltration-dialysis. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(19)70742-x
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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