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Estimation of the percentage of free steroid in undiluted serum by centrifugal ultrafiltration-dialysis.
Open accessAbstract
A new method is described for the estimation of the percentages of free steroids in undiluted serum or plasma. Samples were incubated with 3H-steroid and [14C]glucose, and duplicate aliquots of the mixture were subjected to centrifugal ultrafiltration through a dialysis membrane at 37 degrees C. The percentages of free estradiol, testosterone, and progesterone in serum were estimated by comparing the ratio of 3H-steroid to [14C]glucose in the ultrafiltrate with the corresponding ratio in the serum retained by the dialysis membrane. In addition to being highly reproducible, the present method affords several advantages over existing methods. The use of [14C]glucose to monitor the movement of unbound serum components obviates measurement of the ultrafiltrate volume, and because the ultrafiltrate remains in contact with the dialysis membrane, small molecular weight components are able to pass through the dialysis membrane in either direction during centrifugal ultrafiltration. This latter feature, which could be described as "isodialysis," allows the estimation of the percentage of free steroid under conditions which closely approximate the in vivo situation.
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Cited by
DUNN, J. F., NISULA, B. C., & RODBARD, D. (1981) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem-53-1-58 · Cited by 1,238
The serum transport of steroid hormones
SIITERI, P. K., MURAI, J. T., RAYMOURE, W. J., KUHN, R. W., HAMMOND, G. L., & NISKER, J. A. (1982) · Proceedings of the 1981 Laurentian Hormone Conference · doi:10.1016/b978-0-12-571138-8.50016-0 · Cited by 770
Self-citationSalivary cortisol: a better measure of adrenal cortical function than serum cortisol
Vining, R. F., McGinley, R. A., Maksvytis, J. J., & Ho, K. Y. (1983) · Annals of Clinical Biochemistry International Journal of Laboratory Medicine · doi:10.1177/000456328302000601 · Cited by 684
Field, A. E., Colditz, G. A., Willett, W. C., Longcope, C., & McKinlay, J. B. (1994) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem.79.5.7962322 · Cited by 421
Plasma steroid-binding proteins: primary gatekeepers of steroid hormone action
Hammond, G. L. (2016) · Journal of Endocrinology · doi:10.1530/joe-16-0070 · Cited by 356
Self-citationLy, L. P., & Handelsman, D. J. (2005) · European Journal of Endocrinology · doi:10.1530/eje.1.01844 · Cited by 180
Predictive accuracy and sources of variability in calculated free testosterone estimates
Sartorius, G., Ly, L. P., Sikaris, K., McLachlan, R., & Handelsman, D. J. (2009) · Annals of Clinical Biochemistry: International Journal of Laboratory Medicine · doi:10.1258/acb.2008.008171 · Cited by 175
The analog free testosterone assay: are the results in men clinically useful?
Winters, S. J., Kelley, D. E., & Goodpaster, B. (1998) · Clinical Chemistry · doi:10.1093/clinchem/44.10.2178 · Cited by 160
Bikle, D. D., Halloran, B. P., Gee, E., Ryzen, E., & Haddad, J. G. (1986) · Journal of Clinical Investigation · doi:10.1172/jci112636 · Cited by 158
Spratt, D. I., Bigos, S. T., Beitins, I., Cox, P., Longcope, C., & Orav, J. (1992) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem.75.6.1464665 · Cited by 118
Liu, P. Y., Wishart, S. M., & Handelsman, D. J. (2002) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem.87.7.8630 · Cited by 112
Oral contraceptives, androgens, and the sexuality of young women: II. The role of androgens
Bancroft, J., Sherwin, B. B., Alexander, G. M., Davidson, D. W., & Walker, A. (1991) · Archives of Sexual Behavior · doi:10.1007/bf01541939 · Cited by 102
Van Uytfanghe, K., Stöckl, D., Kaufman, J. M., Fiers, T., Ross, H. A., De Leenheer, A. P., & Thienpont, L. M. (2004) · Clinical Chemistry · doi:10.1373/clinchem.2004.037358 · Cited by 73
Van Uytfanghe, K., Stöckl, D., Kaufman, J. M., Fiers, T., De Leenheer, A., & Thienpont, L. M. (2005) · Clinical Biochemistry · doi:10.1016/j.clinbiochem.2004.12.001 · Cited by 71
Dowsett, M., Mansfield, M. D., Griggs, D. J., & Jeffcoate, S. L. (1984) · Journal of Steroid Biochemistry · doi:10.1016/0022-4731(84)90290-5 · Cited by 28
DOSAGE‐RELATED EFFECTS OF DANAZOL ON SEX HORMONE BINDING GLOBULIN AND FREE AND TOTAL ANDROGEN LEVELS
FORBES, K. L., DOWSETT, M., ROSE, G. L., MUDGE, J. E., & JEFFCOATE, S. L. (1986) · Clinical Endocrinology · doi:10.1111/j.1365-2265.1986.tb03614.x · Cited by 16
Metadata
- Authors
- Hammond, G. L., Nisker, J. A., Jones, L. A., & Siiteri, P. K.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1980-06
- Volume
- 255
- Issue
- 11
- Pages
- 5023-5026
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1517007955
- Cited by
- 268
- References
- 0
- FWCI
- 6.76
- Type
- journal article
- Language
- en
- Open access
- hybrid
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
