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The effect of activating ligands on the intrinsic fluorescence of guanine nucleotide-binding regulatory proteins.
Open accessAbstract
The intensity of the tryptophan fluorescence of the alpha subunits of guanine nucleotide-binding regulatory proteins increases when they bind guanosine 5'-O-(3-thio)triphosphate (GTY gamma S). The kinetics of the fluorescence enhancement and of the measured binding of [35S]GTP gamma S are well correlated. The addition of Mg2+ to the nucleotide-bound proteins causes a further, rapid increase in the fluorescence intensity. Similar effects result from exposure of the proteins to F- and Mg2+, and the required concentration of F- is reduced by the inclusion of Al3+. It is presumed that the more highly fluorescent state of the G protein alpha subunits represents their active conformation.
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Citations
Cited by
Ross, E. M., & Wilkie, T. M. (2000) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.69.1.795 · Cited by 1,156
Self-citationReceptor-effector coupling by G proteins
Birnbaumer, L., Abramowitz, J., & Brown, A. M. (1990) · Biochimica et Biophysica Acta (BBA) - Reviews on Biomembranes · doi:10.1016/0304-4157(90)90007-y · Cited by 1,118
Higashijima, T., Ferguson, K. M., Sternweis, P. C., Smigel, M. D., & Gilman, A. G. (1987) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)75851-7 · Cited by 366
Self-citationAluminofluoride and beryllofluoride complexes: new phosphate analogs in enzymology
Chabre, M. (1990) · Trends in Biochemical Sciences · doi:10.1016/0968-0004(90)90117-t · Cited by 310
Fluoride is not an activator of the smaller (20-25 kDa) GTP-binding proteins
Kahn, R. A. (1991) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)98446-2 · Cited by 267
Metadata
- Authors
- Higashijima, T., Ferguson, K. M., Sternweis, P. C., Ross, E. M., Smigel, M. D., & Gilman, A. G.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1987-01
- Volume
- 262
- Issue
- 2
- Pages
- 752-756
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2133226609
- Cited by
- 188
- References
- 0
- FWCI
- 4.23
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Higashijima, T., Ferguson, K. M., Sternweis, P. C., Ross, E. M., Smigel, M. D., & Gilman, A. G. (1987). The effect of activating ligands on the intrinsic fluorescence of guanine nucleotide-binding regulatory proteins. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(19)75849-9
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