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A Specific and Sensitive Assay for Disulfides
Open accessAbstract
A specific and sensitive assay for disulfide groups has been developed, based on reduction with dithioerythritol or dithiothreitol and determination of the resulting monothiols with 5,5′-dithiobis(2-nitrobenzoic acid) in the presence of arsenite. The dissociation constants for the complexes formed between arsenite and dithioerythritol, dithiothreitol, 1,3-dithioglycerol, and 1,2-dithioglycerol have been measured, as well as the rate constants for the formation and breakdown of the complexes and for the bimolecular reaction between the complexes and 5,5′-dithiobis(2-nitrobenzoic acid). The pK values of dithiothreitol are 8.3 and 9.5, and those for dithioerythritol are 9.0 and 9.9.
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Metadata
- Authors
- Zahler, W. L., & Cleland, W. W.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1968-02
- Volume
- 243
- Issue
- 4
- Pages
- 716-719
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1923027407
- Cited by
- 445
- References
- 0
- FWCI
- 5.18
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Zahler, W. L., & Cleland, W. W. (1968). A Specific and Sensitive Assay for Disulfides. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(19)81724-6
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