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Purification of a protein cofactor required for ADP-ribosylation of the stimulatory regulatory component of adenylate cyclase by cholera toxin.
Open accessAbstract
A factor (ARF) that is required for the cholera toxin-dependent ADP-ribosylation of the stimulatory, GTP-binding regulatory component (Gs) of adenylate cyclase has been purified about 2000-fold from cholate extracts of rabbit liver membranes. ARF is an intrinsic membrane protein with Mr = 21,000. The final product can be resolved into two polypeptides with very similar molecular weights; each of these has ARF activity. The ADP-ribosylation of Gs can now be studied with defined components. GTP and ARF are both necessary cofactors. The data imply that the substrates for the activated toxin are NAD and a GTP X Gs X ARF complex, and the reaction proceeds in a lipid environment. The apparent ability of ARF to bind to the alpha subunit of Gs suggests that it may play another, unknown role in the regulation of adenylate cyclase activity.
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Cited by
Receptor-effector coupling by G proteins
Birnbaumer, L., Abramowitz, J., & Brown, A. M. (1990) · Biochimica et Biophysica Acta (BBA) - Reviews on Biomembranes · doi:10.1016/0304-4157(90)90007-y · Cited by 1,118
Ras history: The saga continues
Cox, A. D., & Der, C. J. (2010) · Small GTPases · doi:10.4161/sgtp.1.1.12178 · Cited by 706
Phospholipase D: a Downstream Effector of ARF in Granulocytes
Cockcroft, S., Thomas, G. M. H., Fensome, A., Geny, B., Cunningham, E., Gout, I., Hiles, I., Totty, N. F., Truong, O., & Hsuan, J. J. (1994) · Science · doi:10.1126/science.8290961 · Cited by 631
Human RAS Superfamily Proteins and Related GTPases
Colicelli, J. (2004) · Science's STKE · doi:10.1126/stke.2502004re13 · Cited by 582
REGULATION OF EUKARYOTIC PHOSPHATIDYLINOSITOL-SPECIFIC PHOSPHOLIPASE C AND PHOSPHOLIPASE D
Singer, W. D., Brown, H. A., & Sternweis, P. C. (1997) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.66.1.475 · Cited by 401
Metadata
- Authors
- Kahn, R. A., & Gilman, A. G.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1984-05
- Volume
- 259
- Issue
- 10
- Pages
- 6228-6234
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1535671912
- Cited by
- 415
- References
- 0
- FWCI
- 3.44
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Kahn, R. A., & Gilman, A. G. (1984). Purification of a protein cofactor required for ADP-ribosylation of the stimulatory regulatory component of adenylate cyclase by cholera toxin. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(20)82130-9
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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