Development of a fluorescent antibody virus neutralisation test (FAVN test) for the quantitation of rabies-neutralising antibody
Abstract
A microtest named the FAVN test (fluorescent antibody virus neutralisation test), which is an adaptation of the original rapid fluorescent focus inhibition test (RFFIT) has been developed and evaluated. One hundred percent specificity was estimated using 414 sera from dogs sampled in rabies-free areas or from non-vaccinated animals. The accuracy as determined by the agreement between observed and expected values using sera of known titres was satisfactory. Serum samples from unvaccinated and vaccinated dogs (using sera with titres near 0.5 IU/ml) were assayed for rabies antibody by the FAVN test, the RFFIT and the mouse neutralisation test (MNT): comparative results obtained on the same sera with the three tests showed good agreement. Furthermore, distinguishing negative sera from positive sera with low titres is much easier with the FAVN test than with the RFFIT.
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Anti-rabies serum antibodies were analyzed by FAVN test according to WHO protocol.
“Anti-rabies serum antibodies were analyzed by FAVN test by Eurovir Hygiene-Institut, Luckenwalde, Germany according to WHO protocol [30].”
The RABV neutralization assay was conducted in a certified BSL-2 laboratory using the FAVN assay with WHO international standard immunoglobulin and naïve serum as positive and negative controls.
“The RABV neutralization assay was conducted in a certified BSL-2 laboratory. Sera were analyzed for VNAs using the FAVN assay, with RABV (CVS-11 strain) as the test virus and BHK-21 cells as the host cells ( 52 ). The calibrated World Health Organization international standard immunoglobulin (second human rabies immunoglobulin preparation, National Institute for Standards and Control, Potters Bar,”
From Ecological vaccination: A strategy to prevent zoonotic spillover from bats (2026) · cites this paper as [52]
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Schnee, M., Vogel, A. B., Voss, D., Petsch, B., Baumhof, P., Kramps, T., & Stitz, L. (2016) · PLOS Neglected Tropical Diseases · doi:10.1371/journal.pntd.0004746 · Cited by 212 · see citing works
“Antirabies serum antibodies were analyzed by FAVN test by Eurovir Hygiene-Institut, Luckenwalde, Germany according to WHO protocol [30].”
MethodA thermostable messenger RNA based vaccine against rabies
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Development of broad‐spectrum human monoclonal antibodies for rabies post‐exposure prophylaxis
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Ecological vaccination: A strategy to prevent zoonotic spillover from bats
Li, H., Yuan, F., Yao, Y., Wang, J., Li, C., Liu, Y., Yang, J., Zhang, Z., Liu, J., Liu, J., et al. (2026) · Science Advances · doi:10.1126/sciadv.aec0269
- Authors
- Cliquet, F., Aubert, M., & Sagné, L.
- Corresponding author
- Cliquet, F.
- ORCID
- Cliquet, F.
- Venue
- Journal of Immunological Methods
- Publisher
- Elsevier BV
- Published
- 1998-01
- Volume
- 212
- Issue
- 1
- Pages
- 79-87
- ISSN
- 0022-1759 (ISSN-L)
- OpenAlex
- W1989888538
- Cited by
- 432
- References
- 13
- Citations by year
- 2026: 14 · 2025: 20 · 2024: 24 · 2023: 20 · 2022: 24 · 2021: 25 · 2020: 17 · 2019: 17 · 2018: 19 · 2017: 33 · 2016: 24 · 2015: 19 · 2014: 21 · 2013: 12 · 2012: 15
- FWCI
- 0.59
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Immunological Methods (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Immunology and Microbiology › Virology › Rabies epidemiology and control
- Health Sciences › Medicine › Epidemiology › Virology and Viral Diseases
- Life Sciences › Agricultural and Biological Sciences › Agronomy and Crop Science › Animal Disease Management and Epidemiology
- Keywords
- rabies, rapid fluorescent focus inhibition test, antibody quantitation, dog vaccination, test specificity, diagnostic accuracy, serological diagnosis
- MeSH terms
- Animals, Antibodies, Viral, Dogs, Neutralization Tests, Rabies, Rabies virus, Reference Standards, Sensitivity and Specificity, Reproducibility of Results
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