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Cited by 324Fatty Acid Research and HealthLipid metabolism and biosynthesisCardiovascular Function and Risk Factors

The DOI matches its Crossref record; 47 of the 47 references it cites were found in a registry or an index.

Saturated but not mono-unsaturated fatty acids induce apoptotic cell death in neonatal rat ventricular myocytes

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de Vries, J E, Vork, M M, Roemen, T H, de Jong, Y F, Cleutjens, J P, van der Vusse, G J, van Bilsen, M·1997·Journal of Lipid Research
DOI 10.1016/s0022-2275(20)37421-6
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Abstract

The energy need of cardiac muscle cells in vivo is largely covered by the oxidation of saturated and mono-unsaturated fatty acids (FA). However, in vitro studies have shown that the saturated FA C16:0 at physiological concentrations exerts detrimental effects on primary cultures of neonatal rat ventricular myocytes by, as yet, unknown mechanisms. To evaluate the noxious effects of FA in more detail, neonatal cardiomyocytes were exposed to saturated (C16:0; C18:0) or mono-unsaturated (C16:1; cis-C18:1; trans-C18:1) FA, or combinations thereof for up to 48 h. FA (0.5 mM) complexed to bovine serum albumin (BSA) (0.15 mM) were added to a glucose-containing defined medium. Irrespective of the length and degree of unsaturation of the aliphatic chain, FA supplied to the cells were readily incorporated in the phospholipid pool. In the presence of mono-unsaturated FA, cardiomyocytes remained healthy and accumulated substantial amounts of triacylglycerol. In contrast, within 24 h after application of the saturated FA C16:0 or C18:0, cells had become irreversibly damaged, as evidenced by the presence of pyknotic nuclei and massive release of the cytosolic markers lactate dehydrogenase (LDH) and fatty acid-binding protein (FABP). Moreover, the occurrence of DNA-laddering indicated that apoptosis was involved. Induction of apoptotic cell death by C16:0 was counteracted by the co-administration of equimolar amounts of cis-C18:1, whereas trans-C18:1 delayed, but did not prevent, loss of cardiomyocyte viability. The present findings suggest that the incorporation of saturated, but not mono-unsaturated, fatty acids induces alterations in the phospholipid membrane, which initiate apoptotic cell death in neonatal cardiomyocytes.

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Research works printed but not linked: 2

  1. De Vries, 1995, Saturated, but not mono-unsaturated, fatty acids induce cell death in neonatal rat ventricular cardiomyocytes. Eur, J. Physiol., S430:, R67
  2. Goligan, J. E., A. M. Kruisbeek, D. H. Margulies, E. M. Shevarh, and W. Strober, editors. 1991. Current Protocols for Immunology. Greene Publishing Associates and Wiley-Interscience, New York. 3.17.7-3.17.8.

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Metadata

Authors
de Vries, J. E., Vork, M. M., Roemen, T. H., de Jong, Y. F., Cleutjens, J. P., van der Vusse, G. J., & van Bilsen, M.
Affiliations
Maastricht University (NL)
ORCID
Cleutjens, J P
Venue
Journal of Lipid Research
Publisher
Elsevier BV
Published
1997-07
Volume
38
Issue
7
Pages
1384-1394
ISSN
0022-2275 (ISSN-L)
DOI
10.1016/s0022-2275(20)37421-6
OpenAlex
W2407618083
Cited by
324
References
47
Citations by year
2026: 2 · 2025: 3 · 2024: 8 · 2023: 3 · 2022: 7 · 2021: 12 · 2020: 8 · 2019: 8 · 2018: 9 · 2017: 9 · 2016: 14 · 2015: 9 · 2014: 7 · 2013: 21 · 2012: 16
FWCI
6.75
Type
journal article
Language
en
Open access
Licence
Locations
  • Journal of Lipid Research (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Research Publications (Maastricht University) (publishedVersion, cc-by)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Topics
  • Health Sciences › Nursing › Nutrition and Dietetics › Fatty Acid Research and Health
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
  • Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiovascular Function and Risk Factors
Keywords
saturated fatty acids, monounsaturated fatty acids, palmitic acid, stearic acid, cardiomyocyte apoptosis, neonatal rat ventricular myocytes, membrane damage, triglyceride accumulation, fatty acid oxidation, cardiotoxicity
MeSH terms
Fatty Acid-Binding Protein 7, Animals, Animals, Newborn, Carrier Proteins, Cells, Cultured, Culture Media, Fatty Acids, Fatty Acids, Monounsaturated, Glucose, Kinetics, L-Lactate Dehydrogenase, Myocardium, Neoplasm Proteins, Nerve Tissue Proteins, Phospholipids, Rats, Inbred WKY, Serum Albumin, Bovine, Triglycerides, Apoptosis, Myelin P2 Protein, Fatty Acid-Binding Proteins, Rats

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