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Investigation of plasma membrane-associated apolipoprotein E in primary macrophages

Deng, J, Rudick, V, Rudick, M, Dory, L·1997·Journal of Lipid Research
DOI 10.1016/s0022-2275(20)37435-6
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Abstract

Our previous studies identified the lysosome as the compartment for degradation of newly synthesized apoE in primary macrophages. Lysosomal degradation of newly synthesized apoE is extensive and rapid (> 50% in 60 min). In the present study we tested the hypothesis that the macrophage cell surface is part of the itinerary of apoE in its path to the lysosomes. We therefore examined the existence and size of the apoE pool associated with the macrophage cell surface. Such a pool may not only provide a mechanism of targeting apoE for lysosomal degradation, by endocytosis, but also have important implications for the metabolism of lipoproteins by macrophages. Treatment of macrophages with heparin (10 micrograms/ml and 5 mg/ml) and heparinase I (1 U/ml), which releases substantial amounts of apoE from HepG2 cells, results in no additional release of apoE from macrophages. Treatment of macrophages with xyloside (1 mM) or GRGDTP (500 micrograms/ml) does not decrease the extent of cell-associated apoE. Both immunogold labeling, followed by electron microscopy, and immunofluorescent labeling and light microscopy further confirm the lack of significant amounts of cell surface-associated apoE in macrophages. In contrast, immunolabeled apoE is readily observed in permeabilized cells. Taken together, these data indicate the absence of significant apoE-glycosaminoglycan interaction at the macrophage cell surface. The lack of such an interaction is likely due to paucity of heparan sulfate pr…

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Metadata

Authors
Deng, J., Rudick, V, Rudick, M., & Dory, L.
Venue
Journal of Lipid Research
Publisher
Elsevier BV
Published
1997-02
Volume
38
Issue
2
Pages
217-227
ISSN
0022-2275
DOI
10.1016/s0022-2275(20)37435-6
Cited by
14
References
1
Language
en
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