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Apolipoprotein E localization in rat hepatocytes by immunogold labeling of cryothin sections

Hamilton, RL, Wong, JS, Guo, LS, Krisans, S, Havel, RJ·1990·Journal of Lipid Research
DOI 10.1016/s0022-2275(20)42343-0
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Abstract

The distribution of apolipoprotein (apo) E in rat hepatocytes was investigated with an affinity-purified polyclonal antibody raised against apoE isolated from hepatogeneous very low density lipoproteins (VLDL). The distribution of this antibody was visualized with colloidal gold complexed to anti-rabbit IgG. By epipolarization microscopy, apoE was found uniformly along the basolateral surfaces of all hepatic parenchymal cells, showing a striking intensity along the sinusoidal front. Punctate deposits of colloidal gold appeared to be randomly distributed within all hepatocytes. Widely scattered Kupffer cells also stained for apoE. Electron microscopic examination of immunogold-labeled cryothin sections showed that hepatocytic microvilli projecting into the space of Disse consistently contained clusters of immunogold. The gold particles were variably associated with evident lipoprotein particles, raising the possibility that apoE alone may bind to receptors or other macromolecules at the surface of hepatocytes. Endosomes near the sinusoidal front and multivesicular bodies in the Golgi/biliary area labeled intensely for apoE, consistent with a high content of apoE associated with triglyceride-rich lipoprotein remnants contained within these organelles. Some but not all nascent VLDL particles within putative forming Golgi secretory vesicles were labeled, but many other Golgi vesicles and cisternae that lacked evident VLDL particles were also labeled. These results suggest that at…

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  • Reduced sulfation of heparan chains could also decrease the pool of apoE/Lpl/HL associated with the hepatocyte plasma membrane, which may act as a reservoir for enriching TRLs with these ligands or as molecular bridges between lipoproteins and their receptors.

    “Reduced sulfation of heparan chains could also decrease the pool of apoE/Lpl/HL associated with the hepatocyte plasma membrane (9, 10, 59), which may act as a reservoir for enriching TRLs with these ligands or as molecular bridges between lipoproteins their receptors.”

    From Liver heparan sulfate proteoglycans mediate clearance of triglyceride-rich lipoproteins independently of LDL receptor family members (2007) · cites this paper as [59]

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Research works printed but not linked: 47

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Metadata

Authors
Hamilton, R., Wong, J., Guo, L., Krisans, S., & Havel, R.
Venue
Journal of Lipid Research
Publisher
Elsevier BV
Published
1990-09
Volume
31
Issue
9
Pages
1589-1603
ISSN
0022-2275
DOI
10.1016/s0022-2275(20)42343-0
Cited by
129
References
1
Language
en
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