The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Procedure for determination of free and total cholesterol in micro- or nanogram amounts suitable for studies with cultured cells.
Open accessAbstract
Procedures for the determination of free and total cholesterol in lipid extracts or sonicates of 10(4) cultured human skin fibroblasts are described. The method for free cholesterol employs cholesterol oxidase to generate H2O2 and peroxidase to catalyze the reaction of H2O2 with p-hydroxyphenylacetic acid to yield a stable fluorescent product. Cholesterol ester hydrolase is included for determination of total cholesterol. When samples of sonified cell suspensions are used directly, the extraction of lipids is avoided, permitting one person to carry out analyses of 30 or more subcultures in one day.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Amino acid metabolism in Mammalian cell cultures
Eagle, H. (1959) · Science · doi:10.1126/science.130.3373.432 · Cited by 4,011
Cited by
Krueger, K. E., & Papadopoulos, V. (1990) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)77217-7 · Cited by 345
Ma, K. L., Ruan, X. Z., Powis, S. H., Chen, Y., Moorhead, J. F., & Varghese, Z. (2008) · Hepatology · doi:10.1002/hep.22423 · Cited by 218
Infectious Agents Are Not Necessary for Murine Atherogenesis
Wright, S. D., Burton, C., Hernandez, M., Hassing, H., Montenegro, J., Mundt, S., Patel, S., Card, D. J., Hermanowski-Vosatka, A., Bergstrom, J. D., et al. (2000) · The Journal of Experimental Medicine · doi:10.1084/jem.191.8.1437 · Cited by 176
Cherradi, N., Rossier, M. F., Vallotton, M. B., Timberg, R., Friedberg, I., Orly, J., Wang, X. J., Stocco, D. M., & Capponi, A. M. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.12.7899 · Cited by 175
Hansen, G. H., Immerdal, L., Thorsen, E., Niels-Christiansen, L.-L., Nystrøm, B. T., Demant, E. J. F., & Danielsen, E. M. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m102667200 · Cited by 114
Metadata
- Authors
- Gamble, W., Vaughan, M., Kruth, H. S., & Avigan, J.
- Venue
- Journal of Lipid Research
- Publisher
- Elsevier BV
- Published
- 1978-11
- Volume
- 19
- Issue
- 8
- Pages
- 1068-1070
- ISSN
- 0022-2275 (ISSN-L)
- OpenAlex
- W2165482355
- Cited by
- 557
- References
- 1
- FWCI
- 12.39
- Type
- journal article
- Language
- en
- Open access
- hybrid
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Procedure for determination of free and total cholesterol in micro- or nanogram amounts suitable for studies with cultured cells. https://app.cochat.ai/papers/10.1016/s0022-2275(20)40693-5
- The paper
- Gamble, W., Vaughan, M., Kruth, H. S., & Avigan, J. (1978). Procedure for determination of free and total cholesterol in micro- or nanogram amounts suitable for studies with cultured cells. Journal of Lipid Research. https://doi.org/10.1016/s0022-2275(20)40693-5
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
