The DOI matches its Crossref record.
Re-evaluation of the 3 alpha-hydroxysteroid dehydrogenase assay for total bile acids in bile.
Open accessAbstract
A review of the 3 alpha-hydroxysteroid dehydrogenase method for determining the concentration of total bile acids in bile is described. The optimum conditions for the assay were established with respect to pH, temperature, incubation time, amount of NAD+, and units of enzyme activity required to obtain complete oxidation of the substrate under fixed conditions. Furthermore, the effect of hydrazine hydrate, methanol, and bile volume on the reaction was examined. It was also established that the bile acid concentration in bile samples with a high molar percentage of cholesterol would be overestimated if 3 beta-hydroxysteroid dehydrogenase were present with the 3 alpha-enzyme.
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Cited by
Cholic acid mediates negative feedback regulation of bile acid synthesis in mice
Li-Hawkins, J., Gåfvels, M., Olin, M., Lund, E. G., Andersson, U., Schuster, G., Björkhem, I., Russell, D. W., & Eggertsen, G. (2002) · Journal of Clinical Investigation · doi:10.1172/jci0216309 · Cited by 227
Pizarro, M., Balasubramaniyan, N., Solís, N., Solar, A., Duarte, I., Miquel, J. F., Suchy, F. J., Trauner, M., Accatino, L., Ananthanarayanan, M., et al. (2004) · Gut · doi:10.1136/gut.2003.037689 · Cited by 87
Fat digestion and absorption in spice‐pretreated rats
Prakash, U. N., & Srinivasan, K. (2012) · Journal of the Science of Food and Agriculture · doi:10.1002/jsfa.4597 · Cited by 62
Bennett, A. J., Billett, M. A., Salter, A. M., Mangiapane, E. H., Bruce, J. S., Anderton, K. L., Marenah, C. B., Lawson, N., & White, D. A. (1995) · Biochemical Journal · doi:10.1042/bj3110167 · Cited by 46
Méndez-Sánchez, N., González, V., Aguayo, P., Sánchez, J. M., Tanimoto, M. A., Elizondo, J., & Uribe, M. (2001) · The Journal of Nutrition · doi:10.1093/jn/131.9.2300 · Cited by 41
Metadata
- Authors
- Turley, S. D., & Dietschy, J. M.
- Venue
- Journal of Lipid Research
- Publisher
- Elsevier BV
- Published
- 1978-09
- Volume
- 19
- Issue
- 7
- Pages
- 924-928
- ISSN
- 0022-2275 (ISSN-L)
- OpenAlex
- W1858850028
- Cited by
- 517
- References
- 0
- FWCI
- 5.87
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- CoChat. (2026). Citation check: Re-evaluation of the 3 alpha-hydroxysteroid dehydrogenase assay for total bile acids in bile. https://app.cochat.ai/papers/10.1016/s0022-2275(20)40709-6
- The paper
- Turley, S. D., & Dietschy, J. M. (1978). Re-evaluation of the 3 alpha-hydroxysteroid dehydrogenase assay for total bile acids in bile. Journal of Lipid Research. https://doi.org/10.1016/s0022-2275(20)40709-6
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
