Cited by 99Antioxidant Activity and Oxidative StressRedox biology and oxidative stressErythrocyte Function and Pathophysiology

Role of vitamin E in glutathione-induced oxidant stress: methemoglobin, lipid peroxidation, and hemolysis

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Brownlee, N R, Huttner, J J, Panganamala, R V, Cornwell, D G·1977·Journal of Lipid Research

Abstract

Red blood cells (RBC) from normal and vitamin E-deficient rats were incubated in a hypertonic solution of reduced glutathione adjusted to pH 8. Methemoglobin formation occurred in intact RBC from both normal and vitamin E-deficient rats. Hemolysis was significantly greater in RBC from vitamin E-deficient rats. Experiments with catalase, superoxide dismutase, and methional showed that H(2)O(2) was the primary extracellular source of oxidant stress. Extracellular superoxide and hydroxyl radical were not involved in oxidant stress. Experiments with dimethyl sulfoxide showed that intracellular hydroxyl radical, generated from H(2)O(2), was the hemolytic agent. Neither methemoglobin formation nor lipid peroxidation involved hydroxyl radical. Indeed, lipid peroxidation and hemolysis in RBC from vitamin E-deficient rats were concurrent rather than consecutive events. Phase contrast microscopy showed that rigid, crenated RBC with a precipitate around the interior periphery formed during glutathione-induced oxidant stress. The precipitate dissolved slowly as the crenated RBC were converted to smooth ghosts. It appeared that protein precipitates involving mixed disulfide bonds were reduced and solubilized when extracellular glutathione penetrated the ruptured cell. Comparisons between normal RBC and vitamin E-deficient RBC suggest that vitamin E has little effect on the inward diffusion of extra-cellular H(2)O(2). Vitamin E apparently interacts with different oxidant species derived from intracellular H(2)O(2) in preventing lipid peroxidation and the sulfhydryl group oxidation leading to hemolysis.

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Crossref · 10.1016/s0022-2275(20)41605-0

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Authors
Brownlee, N. R., Huttner, J. J., Panganamala, R. V., & Cornwell, D. G.
Affiliations
The Ohio State University (US)
Venue
Journal of Lipid Research
Publisher
Elsevier BV
Published
1977-09
Volume
18
Issue
5
Pages
635-644
ISSN
0022-2275 (ISSN-L)
OpenAlex
W2125227227
Cited by
99
References
55
Citations by year
2024: 1 · 2023: 3 · 2021: 1 · 2020: 1 · 2019: 1 · 2017: 1 · 2016: 3 · 2013: 2 · 2012: 3
FWCI
10.02
Type
journal article
Language
en
Open access
Locations
  • Journal of Lipid Research (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
Topics
  • Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
  • Health Sciences › Medicine › Physiology › Erythrocyte Function and Pathophysiology
Keywords
vitamin E deficiency, glutathione, oxidative stress, methemoglobin, lipid peroxidation, hemolysis, erythrocytes, hydrogen peroxide, hydroxyl radicals, superoxide, catalase, superoxide dismutase, methionine, sulfhydryl oxidation, cell ghosts
MeSH terms
Animals, Catalase, Chromatography, Gas, Dimethyl Sulfoxide, Erythrocytes, Glutathione, Hemolysis, Lipids, Male, Methemoglobin, Microscopy, Phase-Contrast, Oxygen Consumption, Peroxidases, Superoxide Dismutase, Vitamin E Deficiency, Rats

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