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Gas-liquid chromatographic determination of human fecal bile acids
Open accessAbstract
A method for the determination of total bile acids in human feces that is suitable for routine application is described and discussed. Bile acids are extracted from freeze-dried feces with acetic acid and toluene, in the presence of the internal standard 23-nordeoxycholic acid. After saponification of the extract, bile acids and the internal standard are methylated and converted by mild chromic acid oxidation into their ketonic derivatives. The resultant mixture of a few stable compounds can be separated and measured quantitatively by gas-liquid chromatography on a methylsiloxane polymer. A reference bile acid mixture including the internal standard is also taken through the entire procedure with each series of samples. It has been demonstrated that, in spite of the omission of the usual purification steps, the method is specific for bile acids.
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Citations
Cited by
General methods for the analysis of metabolic profiles of bile acids and related compounds in feces.
Setchell, K. D., Lawson, A. M., Tanida, N., & Sjövall, J. (1983) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)37923-2 · Cited by 285
Diet and faecal flora in three dietary groups in rural northern Nigeria
Drasar, B. S., Montgomery, F., & Tomkins, A. M. (1986) · Journal of Hygiene · doi:10.1017/s0022172400062537 · Cited by 14
Metadata
- Authors
- Evrard, E., & Janssen, G.
- Venue
- Journal of Lipid Research
- Publisher
- Elsevier BV
- Published
- 1968-03
- Volume
- 9
- Issue
- 2
- Pages
- 226-236
- ISSN
- 0022-2275 (ISSN-L)
- OpenAlex
- W2183815026
- Cited by
- 177
- References
- 0
- FWCI
- 4.64
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Evrard, E., & Janssen, G. (1968). Gas-liquid chromatographic determination of human fecal bile acids. Journal of Lipid Research. https://doi.org/10.1016/s0022-2275(20)43123-2
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
