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Cited by 39Adenosine and Purinergic SignalingNeuroscience and Neuropharmacology ResearchPain Mechanisms and Treatments

P2-receptor-mediated inhibition of serotonin release in the rat brain cortex

Von Kügelgen, Ivar, Koch, Helga, Starke, Klaus·1997·Neuropharmacology
DOI 10.1016/s0028-3908(97)00101-9
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Abstract

The possibility of a P2-receptor-mediated modulation of the release of serotonin in the rat brain cortex was investigated in occipito-parietal slices preincubated with [3H]serotonin and then superfused and stimulated electrically (10 pulses, 1 Hz). Adenosine receptor agonists decreased the stimulation-evoked overflow of tritium at best slightly; the selective A1 agonist N6-cyclopentyl-adenosine caused no change. Several nucleotides had more marked effects: ATP (3-1000 microM), adenosine-5'-O-(3-thiotriphosphate) (3-300 microM) and P1,P5-di(adenosine-5')-pentaphosphate (3-300 microM) decreased the evoked overflow by up to ca 35%. AMP, alpha,beta-methylene-ATP and UTP produced smaller decreases and 2-methylthio-ATP and UMP caused no change. The inhibition by ATP was attenuated both by the P1-receptor antagonist 8-(p-sulphophenyl)-theophylline (100 microM) and by the P2-receptor antagonist suramin (300 microM) but was not changed by indomethacin (10 microM) and NG-nitro-L-arginine (10 microM). We conclude that the release of serotonin in the rat brain cortex is inhibited through presynaptic P1-receptors (which are not A1) as well as P2-receptors. Inhibition of release via P2-receptors has been previously shown for noradrenaline (brain cortex and hippocampus) and dopamine (neostriatum) and, hence, may be widespread. Differences between transmitter systems exist, however, in the degree of their sensitivity to presynaptic P2-receptor-mediated modulation.

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  • Activation of presynaptic P2R in rat cortical slices inhibits electrically stimulated 5-HT release.

    “Although there is scarce evidence regarding the localization of P2X7R in monoaminergic terminals, studies conducted with radiolabeled ligands indicate that activation of presynaptic P2R in rat cortical slices inhibits electrically-stimulated 5-HT release [94].”

    From P2X7 Receptor Signaling in Stress and Depression (2019) · cites this paper as [94]

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Authors
Von Kügelgen, I., Koch, H., & Starke, K.
Affiliations
University of Freiburg (DE)
Corresponding author
Starke, Klaus
Venue
Neuropharmacology
Publisher
Elsevier BV
Published
1997-09
Volume
36
Issue
9
Pages
1221-1227
ISSN
0028-3908 (ISSN-L)
DOI
10.1016/s0028-3908(97)00101-9
OpenAlex
W2086787066
Cited by
38
References
38
Citations by year
2023: 3 · 2019: 1 · 2018: 1 · 2017: 2 · 2016: 2 · 2012: 1
FWCI
1.39
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Neuropharmacology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Physiology › Adenosine and Purinergic Signaling
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Health Sciences › Medicine › Physiology › Pain Mechanisms and Treatments
Keywords
purinergic receptors, serotonin release, presynaptic inhibition, cerebral cortex, ATP, adenosine receptors, suramin, adenosine receptor agonists, N6-cyclopentyladenosine
MeSH terms
Adenosine, Adenosine Triphosphate, Animals, Cerebral Cortex, Desipramine, Male, Methiothepin, Quipazine, Serotonin, Serotonin Antagonists, Dinucleoside Phosphates, 2-Chloroadenosine, Rats, Wistar, Selective Serotonin Reuptake Inhibitors, Receptors, Purinergic P2, Adenosine-5'-(N-ethylcarboxamide), Rats, In Vitro Techniques

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