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Cited by 33Nitric Oxide and Endothelin EffectsGout, Hyperuricemia, Uric AcidRedox biology and oxidative stress

Immunoaffinity localization of the enzyme xanthine oxidase on the outside surface of the endothelial cell plasma membrane

Vickers, Selwyn, Schiller, Henry J., Hildreth, James E.K., Bulkley, Gregory B.·1998·Surgery
DOI 10.1016/s0039-6060(98)70102-3
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Abstract

Background Reactive oxygen metabolites generated from endothelial xanthine oxidase (XO) trigger reperfusion injury in many organs. We evaluated the possibility that endothelial XO was localized on the endothelial cell surface, as well as within the cytoplasm.Methods Primary cultures of bovine (BAECs) and porcine (PAECs) aortic endothelial cells were grown in media documented to be free of XO. Polyclonal and monoclonal antibodies were developed against XO. These antibodies were used to evaluate BAEC and PAEC for cell surface XO through immunofluorescence staining, hybridoma cell surface labeling, and endothelial cell surface binding.Results These antibodies bound specifically to the surface of these cells when the membrane was shown to be intact and impermeable (and the cytoplasm inaccessible) to immunoglobulins Moreover, hybridoma cells expressing monoclonal antibody to XO bound specifically to the endothelial cell surface. Finally, intact endothelial cells bound specifically to the anti-XO polyclonal antibodies immobilized to the surface of a Petri dish. The integrity of these endothelial cell plasma membranes was demonstrated by the subsequent growth and replication of these cells in culture.Conclusions These findings indicate that XO is present on the outside surface of the endothelial cell plasma membrane. This would not only explain the known in vivo efficacy of intravascularly administered large molecular weight antioxidants (such as superoxide dismutase) but could have important implications for inflammatory signaling.

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Crossref · 10.1016/s0039-6060(98)70102-3

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38 of 38 references verified0 citation errors · 0 not checkable

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  • Subsequent progress of the atherosclerotic lesion is dependent on immune cells.

    “The current paradigm indicates that macrophage NOX2 is more detrimental in the development of cardiovascular disease (395, 876), with endothelial NOX2 probably involved in the initiation, but not so much in the development of the disease, and a subsequent progress of the atherosclerotic lesion dependent on immune cells (243, 573, 976).”

    From Sources of Vascular Nitric Oxide and Reactive Oxygen Species and Their Regulation (2019) · cites this paper as [976]

  • Mice combining the mutation of p47phox−/− with ApoE−/− appear to be protected from atherosclerotic lesions compared with ApoE−/− mice.

    “The mice combining the mutation of p47phox−/− with ApoE−/− appear to be protected from atherosclerotic lesions (253) compared with ApoE−/− mice (64, 976), although some reports do not find significant differences in atherosclerosis progression or lesion size (443).”

    From Sources of Vascular Nitric Oxide and Reactive Oxygen Species and Their Regulation (2019) · cites this paper as [976]

  • Endothelial NOX2 is probably involved in the initiation, but not so much in the development of cardiovascular disease.

    “The current paradigm indicates that macrophage NOX2 is more detrimental in the development of cardiovascular disease (395, 876), with endothelial NOX2 probably involved in the initiation, but not so much in the development of the disease, and a subsequent progress of the atherosclerotic lesion dependent on immune cells (243, 573, 976).”

    From Sources of Vascular Nitric Oxide and Reactive Oxygen Species and Their Regulation (2019) · cites this paper as [976]

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Authors
Vickers, S., Schiller, H. J., Hildreth, J. E. K., & Bulkley, G. B.
ORCID
Vickers, Selwyn
Venue
Surgery
Publisher
Elsevier BV
Published
1998-09
Volume
124
Issue
3
Pages
551-560
ISSN
0039-6060 (ISSN-L)
DOI
10.1016/s0039-6060(98)70102-3
OpenAlex
W4300925402
Cited by
33
References
38
Citations by year
2025: 1 · 2023: 1 · 2022: 2 · 2021: 1 · 2019: 2 · 2018: 2 · 2017: 2 · 2016: 1 · 2015: 2 · 2014: 3
FWCI
1.05
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
Surgery (publishedVersion)
Funders
National Institute of Diabetes and Digestive and Kidney Diseases (DK-31764)
Topics
  • Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
  • Health Sciences › Medicine › Nephrology › Gout, Hyperuricemia, Uric Acid
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
Keywords
xanthine oxidase, endothelial cells, plasma membrane, cell surface localization, reactive oxygen species, ischemia-reperfusion injury, monoclonal antibodies, polyclonal antibodies, hybridoma, aortic endothelial cells, superoxide dismutase, antioxidants, inflammatory signaling

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